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AB238855

Anti-TTDA antibody

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(1 Publication)

Rabbit Polyclonal TTDA antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GTF2H5.

View Alternative Names

C6orf175, TTDA, GTF2H5, General transcription factor IIH subunit 5, General transcription factor IIH polypeptide 5, TFB5 ortholog, TFIIH basal transcription factor complex TTD-A subunit, TFIIH subunit p8

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TTDA antibody (AB238855)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTDA antibody (AB238855)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for TTDA (Green) using ab238855 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTDA antibody (AB238855)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTDA antibody (AB238855)

Paraffin-embedded human kidney tissue stained for TTDA using ab238855 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTDA antibody (AB238855)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTDA antibody (AB238855)

Paraffin-embedded human brain tissue stained for TTDA using ab238855 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GTF2H5.

Q6ZYL4

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Complementary Products

Proteins & Peptides

AB187616

Recombinant Human TTDA protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TTDA protein (His tag N-Terminus) (AB187616)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTDA also known as GTF2H5 or TFB5 is a small protein with a mass of approximately 8.4 kDa. It functions as a part of the transcription factor IIH (TFIIH) complex which is essential in DNA repair and transcription. TTDA is expressed in various tissues throughout the body with notable expression in actively dividing cells. Mechanically TTDA interacts with other components within the TFIIH complex to stabilize its structure and support its activities during transcription and nucleotide excision repair (NER).
Biological function summary

This protein plays a role in maintaining genomic integrity by ensuring effective DNA repair. Acting as part of the TFIIH complex TTDA facilitates the recognition and excision of damaged DNA segments through the NER process. It also contributes to the transcription initiation process by unwinding DNA and permitting the RNA polymerase to access the DNA template. These roles are critical for cellular processes like replication and transcription which require accurate and clean DNA templates.

Pathways

TTDA is important in the nucleotide excision repair and transcription initiation pathways. Within the NER pathway TTDA works alongside other TFIIH complex members such as XPB and XPD which helicases that help unwind DNA. In transcription initiation TTDA is related to the functioning of RNA polymerase II ensuring successful transcription starts. The interconnection of TTDA with these processes highlights its contribution to cellular health and replication fidelity.

Mutations or dysfunctions regarding TTDA can lead to conditions such as trichothiodystrophy and xeroderma pigmentosum. These disorders arise from impaired DNA repair mechanisms due to faulty TFIIH complex activities leading to increased sensitivity to UV light and abnormal skin and hair development. TTDA's interaction with other NER proteins like XPD suggests its involvement in maintaining a robust response to DNA damage and its malfunction can contribute to the onset of these genetic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. Necessary for the stability of the TFIIH complex and for the presence of normal levels of TFIIH in the cell.
See full target information GTF2H5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:815 PubMed39528445

2024

GNG5 is a novel regulator of Aβ42 production in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyuan Li,Yan Yang,Shiqi Luo,Wenying Qiu,Xia Wang,Wei Ge
View all publications
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Product promise

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