Rabbit Polyclonal TTF1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
Associated Products
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Target data
Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Alternative names
NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP
Recommended products
Rabbit Polyclonal TTF1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
ab125650 is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of TFF1 are known to exist; ab125650 will detect all three.
- Concentration
- Purification notes
ab125650 is affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
- Biological function summary
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
- Pathways
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
- Associated diseases and disorders
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody (ab125650)
Immunohistochemistry of TTF1/Nkx2-1 in Human lung tissue with ab125650 at 2.5 μg/mL.
Immunocytochemistry/ Immunofluorescence - Anti-TTF1/Nkx2-1 antibody (ab125650)
Immunofluorescence of TTF1/Nkx2-1 in Human lung tissue with ab125650 at 20 ug/mL.
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