Anti-TTF1/Nkx2-1 antibody [EP1584Y]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC

Lab

Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid labelling TTF1 with ab76013 at a concentration of 0.1 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32 mins.

ab76013 anti-TTF1 [EP1584Y] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

AbReview32743****

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Blocked with 5% milk

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) at 1/2000 dilution

All lanes:

HEK293 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

• WB

Lab

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) at 1/10000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

• WB

Lab

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Different batches of ab76013 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.05 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013)

Predicted band size: 39 kDa

false

• WB

Lab

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) at 1/2000 dilution

All lanes:

Rat lung lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

• WB

Lab

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) at 1/2000 dilution

All lanes:

Mouse lung lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

• WB

CiteAb

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (AB76013)

TTF1/Nkx2-1 western blot using anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013. Publication image and figure legend from Fukui, E., Funaki, S., et al., 2019, Stem Cells Int, PubMed 31281377.

ab76013 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76013 please see the product overview.

Differentiation into trilineage cells and alveolar epithelial cells of adipose-derived stem cells (ADSCs). (a) Histological analysis of differentiation of ADSCs into adipogenic, osteogenic, and chondrogenic lineages. Adipogenic differentiation was assessed with oil red O staining. Osteogenic differentiation was examined using alkaline phosphatase staining. Chondrogenic differentiation of ADSCs was examined by alcian blue staining. ADSCs have the capacity to differentiate into each mesenchymal lineage. Scale bars : 50 μm. (b) Western blot analysis of ADSCs. Expression of TTF-1, Pro SPB, and SPC is shown. MLE-12 is a murine lung epithelial cell line. (c) Real-time quantitative RT-PCR analysis of ADSCs. ADSCs were cultured in small airway growth medium (SAGM) or with the stepwise protocol for 28 days. Expression of EPCAM and Cdh-1 (epithelial markers) and Nkx2-1, Sftpb, and Sftpc (type 2 alveolar epithelial cell markers) was analyzed with RT-PCR on day 28 (n = 3). Each value was normalized to the level of Gapdh. ND : not detected. (d) Immunofluorescence staining of ADSCs with anti-EPCAM, anti-TTF-1, anti-SPB, and anti-SPC. Control : ADSCs cultured in growth medium; SAGM : ADSCs cultured in SAGM; stepwise prc : ADSCs cultured with the stepwise protocol. Scale bars : 100 μm. (e) Transmission electron microscopy of ADSCs. Lamellar body-like structures were observed in ADSCs cultured in SAGM or with the stepwise protocol for 28 days. Scale bars : 2 μm.

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