Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 is a rabbit monoclonal antibody that is used in TTF1/Nkx2-1 western blotting and IHC. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1584Y has been tried and trusted by researchers since 2009 and is cited in >210 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | Flow Cyt | WB | ICC/IF | |
---|---|---|---|---|
Human | Tested | Not recommended | Tested | Not recommended |
Mouse | Tested | Not recommended | Tested | Not recommended |
Rat | Tested | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes For unpurified use at 1/500- 1/1000. |
Species Rat | Dilution info 1/2000 | Notes For unpurified use at 1/500- 1/1000. |
Species Human | Dilution info 1/2000 | Notes For unpurified use at 1/500- 1/1000. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
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Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP
Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 is a rabbit monoclonal antibody that is used in TTF1/Nkx2-1 western blotting and IHC. Suitable for human, mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1584Y has been tried and trusted by researchers since 2009 and is cited in >210 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP1584Y
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/10000 dilution
All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
Different batches of ab76013 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.05 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.
All lanes: Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)
All lanes: Rat lung lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
All lanes: Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution
All lanes: Mouse lung lysate at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
Blocked with 5% milk
All lanes: Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution
All lanes: HEK293 whole cell lysate at 30 µg
All lanes: HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa
Exposure time: 2min
Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid labelling TTF1 with ab76013 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32 mins.
ab76013 anti-TTF1 [EP1584Y] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
Image collected and cropped by CiteAb under a CC-BY license from the publication
TTF1/Nkx2-1 western blot using anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013. Publication image and figure legend from Fukui, E., Funaki, S., et al., 2019, Stem Cells Int, PubMed 31281377.
ab76013 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76013 please see the product overview.
Differentiation into trilineage cells and alveolar epithelial cells of adipose-derived stem cells (ADSCs). (a) Histological analysis of differentiation of ADSCs into adipogenic, osteogenic, and chondrogenic lineages. Adipogenic differentiation was assessed with oil red O staining. Osteogenic differentiation was examined using alkaline phosphatase staining. Chondrogenic differentiation of ADSCs was examined by alcian blue staining. ADSCs have the capacity to differentiate into each mesenchymal lineage. Scale bars: 50 μm. (b) Western blot analysis of ADSCs. Expression of TTF-1, Pro SPB, and SPC is shown. MLE-12 is a murine lung epithelial cell line. (c) Real-time quantitative RT-PCR analysis of ADSCs. ADSCs were cultured in small airway growth medium (SAGM) or with the stepwise protocol for 28 days. Expression of EPCAM and Cdh-1 (epithelial markers) and Nkx2-1, Sftpb, and Sftpc (type 2 alveolar epithelial cell markers) was analyzed with RT-PCR on day 28 (n = 3). Each value was normalized to the level of Gapdh. ND: not detected. (d) Immunofluorescence staining of ADSCs with anti-EPCAM, anti-TTF-1, anti-SPB, and anti-SPC. Control: ADSCs cultured in growth medium; SAGM: ADSCs cultured in SAGM; stepwise prc: ADSCs cultured with the stepwise protocol. Scale bars: 100 μm. (e) Transmission electron microscopy of ADSCs. Lamellar body-like structures were observed in ADSCs cultured in SAGM or with the stepwise protocol for 28 days. Scale bars: 2 μm.
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