Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)

This IHC data was generated using the same anti-TTF1/Nkx2-1 antibody clone, EP1584Y, in a different buffer formulation (cat# ab76013).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76013).

• IHC

Lab

Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76013).

Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid labelling TTF1 with ab76013 at a concentration of 0.1 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32 mins.

ab76013 anti-TTF1 [EP1584Y] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76013).

• WB

Lab

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)

This data was developed using ab76013, the same antibody clone in a different buffer formulation. Different batches of ab76013 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.05 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (<a href='/en-us/products/primary-antibodies/ttf1-nkx2-1-antibody-ep1584y-ab76013'>ab76013</a>)

Predicted band size: 39 kDa

false