Rabbit Recombinant Monoclonal TTF1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples. Cited in 19 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89513.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)")
![Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--western-blot-img165371.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54537.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40287.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)")
![Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-img439829.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (AB216648)")
Publications
Filter by
- The Journal of neuroscience : the official journal of the Society for Neuroscience 35:12869-892015Prox1 Regulates the Subtype-Specific Development of Caudal Ganglionic Eminence-Derived GABAergic Cortical Interneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGoichi Miyoshi,Allison Young,Timothy Petros,Theofanis Karayannis,Melissa McKenzie Chang,Alfonso Lavado,Tomohiko Iwano,Miho Nakajima,Hiroki Taniguchi,Z Josh Huang,Nathaniel Heintz,Guillermo Oliver,Fumio Matsuzaki,Robert P Machold,Gord FishellPubMed 26377473
- Scientific reports 5:122582015Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium.
- Development (Cambridge, England) 142:1267-782015Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A Tyson,Ethan M Goldberg,Asif M Maroof,Qing Xu,Timothy J Petros,Stewart A AndersonPubMed 25804737
- European journal of cardio-thoracic surgery : offi 46:e103-122014Lung mesenchymal cells function as an inductive microenvironment for human lung cancer propagating cells†.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Ampollini et. al.PubMed 25312525
- Cancer research 74:5978-882014Modeling lung cancer evolution and preclinical response by orthotopic mouse allografts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Ambrogio et. al.PubMed 25217522
- Cerebral cortex (New York, N.Y. : 1991) 26:131-432014The Role of Sonic Hedgehog in the Specification of Human Cortical Progenitors In Vitro.
- Frontiers in neuroanatomy 8:822014The complexity of the calretinin-expressing progenitors in the human cerebral cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNevena V Radonjić et. al.PubMed 25165435
- Cell death & disease 5:e13112014Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice.
- Cancer discovery 4:781-92014Obligate progression precedes lung adenocarcinoma dissemination.Applications:IHCReactive species:Unspecified reactive speciesDeborah R Caswell et. al.PubMed 24740995
- Proceedings of the National Academy of Sciences of 111:E1723-302014Generation of multiciliated cells in functional airway epithelia from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy L Firth et. al.PubMed 24706852
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
1 product for Alternative Version
Target data
Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Alternative names
NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP
Recommended products
Rabbit Recombinant Monoclonal TTF1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples. Cited in 19 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1584Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab216648 is the carrier-free version of Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
- Biological function summary
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
- Pathways
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
- Associated diseases and disorders
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89513.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013).
![Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--western-blot-img165371.jpg "Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)")
Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)
This data was developed using Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013, the same antibody clone in a different buffer formulation. Different batches of Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.05 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 40 kDa.
All lanes: Western blot - Anti-TTF1/Nkx2-1 antibody [EP1584Y] (Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013)
Predicted band size: 39 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54537.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1/Nkx2-1 with purified Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40287.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)
This IHC data was generated using the same anti-TTF1/Nkx2-1 antibody clone, EP1584Y, in a different buffer formulation (cat# Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1/Nkx2-1 with purified Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
![Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-ep1584y-bsa-and-azide-free-ab216648--immunohistochemistry-img439829.jpg "Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)")
Immunohistochemistry - Anti-TTF1/Nkx2-1 antibody [EP1584Y] - BSA and Azide free (ab216648)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013).
Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid labelling TTF1 with Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32 mins.
Anti-TTF1/Nkx2-1 antibody [EP1584Y] ab76013 anti-TTF1 [EP1584Y] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com