Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) is a rabbit monoclonal antibody detecting TTF1/Nkx2-1 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP
Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) is a rabbit monoclonal antibody detecting TTF1/Nkx2-1 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes: Western blot - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Human fetal lung lysate at 10 µg
Lane 3: Rat lung lysate at 10 µg
Lane 4: Mouse lung lysate at 10 µg
All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 39 kDa
Immunohistochemical analysis of paraffin embedded Human papillary carcinoma of thyroid gland tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemical analysis using ab133737 showing negative staining in human Normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemical analysis using ab133737 showing negative staining in human Glioma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemical analysis using ab133737 showing negative staining in human Breast carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemical analysis using ab133737 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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