AB133737

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]

RabMAb
Recombinant
20ul selling size
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(24 Publications)

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) is a rabbit monoclonal antibody detecting TTF1/Nkx2-1 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis using ab133737 showing negative staining in human Glioma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis using ab133737 showing negative staining in human Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis using ab133737 showing negative staining in human Breast carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis of paraffin embedded Human papillary carcinoma of thyroid gland tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis using ab133737 showing positive staining in human Lung adenocarcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

WB

Unknown

Western blot - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human fetal lung lysate at 10 µg

Lane 3:

Rat lung lysate at 10 µg

Lane 4:

Mouse lung lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 39 kDa

false

OI-RD Scanning - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (AB133737)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]
Carrier free

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5955(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of TTF1/Nkx2-1?
Anti-TTF1/Nkx2-1 [EPR5955(2)] (ab133737) specifically detects a band for TTF1/Nkx2-1 (UniProt: P43699) at a molecular weight of 39kDa.

Trusted by the scientific community
Anti-TTF1/Nkx2-1 [EPR5955(2)] (ab133737) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5955(2)] also available for your convenience: ab133737, Alexa Fluor® 488 - ab206726, Alexa Fluor® 647 - ab206727, Carrier free - ab227574

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.

Biological function summary

TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.

Pathways

The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.

TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

See full target information NKX2-1

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40327401

2025

Tumor suppressors in Sox2-mediated lung cancers promote distinct cell-intrinsic and immunologic remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Nisitha Sengottuvel,Kristina M Whately,Jennifer L Modliszewski,Rani S Sellers,William D Green,Weida Gong,Allison T Woods,Eric W Livingston,Katerina D Fagan-Solis,Gabrielle Cannon,Lincy Edatt,Hong Yuan,Aaron C Chack,Yazmin Sanchez,Katherine Zhou,Alyaa Dawoud,Jarred M Green,Virginia Godfrey,J Justin Milner,Gaorav P Gupta,Chad V Pecot

Molecular cancer therapeutics 23:1626-1638 PubMed39186309

2024

SC134-TCB Targeting Fucosyl-GM1, a T Cell-Engaging Antibody with Potent Antitumor Activity in Preclinical Small Cell Lung Cancer Models.

Applications

Unspecified application

Species

Unspecified reactive species

Foram Dave,Poonam Vaghela,Bryony Heath,Zuzana Dunster,Elena Dubinina,Dhruma Thakker,Katie Mann,Joe Chadwick,Gaëlle Cane,Bubacarr G Kaira,Omar J Mohammed,Ruhul Choudhury,Samantha Paston,Tina Parsons,Mireille Vankemmelbeke,Lindy Durrant

Nature communications 15:4148 PubMed38755149

2024

CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair.

Applications

Unspecified application

Species

Unspecified reactive species

Dalia Hassan,Jichao Chen

iScience 26:107525 PubMed37646018

2023

Novel human pluripotent stem cell-derived hypothalamus organoids demonstrate cellular diversity.

Applications

Unspecified application

Species

Unspecified reactive species

Lily Sarrafha,Drew R Neavin,Gustavo M Parfitt,Ilya A Kruglikov,Kristen Whitney,Ricardo Reyes,Elena Coccia,Tatyana Kareva,Camille Goldman,Regine Tipon,Gist Croft,John F Crary,Joseph E Powell,Joel Blanchard,Tim Ahfeldt

Laboratory investigation; a journal of technical methods and pathology 103:100197 PubMed37307952

2023

Alveolar Mitochondrial Quality Control During Acute Respiratory Distress Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan D Kraft,Elizabeth N Pavlisko,Victor L Roggli,Claude A Piantadosi,Hagir B Suliman

Science signaling 15:eabj4009 PubMed36194647

2022

The kinase PLK1 promotes the development of /-mutant lung adenocarcinoma through transcriptional activation of the receptor RET.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Kong,Derek B Allison,Qiongsi Zhang,Daheng He,Yuntong Li,Fengyi Mao,Chaohao Li,Zhiguo Li,Yanquan Zhang,Jianlin Wang,Chi Wang,Christine F Brainson,Xiaoqi Liu

Developmental cell 57:1866-1882.e10 PubMed35835117

2022

FoxA1 and FoxA2 control growth and cellular identity in NKX2-1-positive lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Orstad,Gabriela Fort,Timothy J Parnell,Alex Jones,Chris Stubben,Brian Lohman,Katherine L Gillis,Walter Orellana,Rushmeen Tariq,Olaf Klingbeil,Klaus Kaestner,Christopher R Vakoc,Benjamin T Spike,Eric L Snyder

Oncogene 41:3423-3432 PubMed35577980

2022

TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Samera H Hamad,Stephanie A Montgomery,Jeremy M Simon,Brittany M Bowman,Kyle B Spainhower,Ryan M Murphy,Erik S Knudsen,Suzanne E Fenton,Scott H Randell,Jeremiah R Holt,D Neil Hayes,Agnieszka K Witkiewicz,Trudy G Oliver,M Ben Major,Bernard E Weissman

Frontiers in molecular neuroscience 15:889922 PubMed35600077

2022

Buprenorphine Exposure Alters the Development and Migration of Interneurons in the Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Vanesa Nieto-Estévez,Jennifer J Donegan,Courtney L McMahon,Hannah B Elam,Teresa A Chavera,Parul Varma,Kelly A Berg,Daniel J Lodge,Jenny Hsieh

Journal of Cancer 13:1468-1479 PubMed35371324

2022

Compared with High-intensity Interval Exercise, Moderate Intensity Constant Load Exercise is more effective in curbing the Growth and Metastasis of Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Ge,Shan Wu,Zhengtang Qi,Shuzhe Ding

View all publications

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Product promise

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