AB227574

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal TTF1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemical analysis using ab133737 showing negative staining in Glioma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemical analysis using ab133737 showing negative staining in Normal tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

This IHC data was generated using the same anti-TTF1/Nkx2-1 antibody clone, EPR5955(2), in a different buffer formulation (cat# ab133737).

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemical analysis using ab133737 showing negative staining in Breast carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemical analysis using ab133737 showing positive staining in Thyroid gland tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

This IHC data was generated using the same anti-TTF1/Nkx2-1 antibody clone, EPR5955(2), in a different buffer formulation (cat# ab133737).

Immunohistochemical analysis of paraffin embedded Human papillary carcinoma of thyroid gland tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemical analysis using ab133737 showing positive staining in Lung adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemical analysis using ab133737 showing positive staining in Thyroid gland carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

OI-RD Scanning - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TTF1/Nkx2-1 antibody [EPR5955(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5955(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab227574 is the carrier-free version of ab133737.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.

Biological function summary

TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.

Pathways

The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.

TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

See full target information NKX2-1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 4:100777 PubMed38744289

2024

Effective cryopreservation of human brain tissue and neural organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Xue,Huijuan Li,Jinhong Xu,Xiao Yu,Linlin Liu,Huihui Liu,Rui Zhao,Zhicheng Shao

International journal of biological sciences 13:652-659 PubMed28539837

2017

Ezh2 Acts as a Tumor Suppressor in Kras-driven Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxiao Wang,Ning Hou,Xuan Cheng,Jishuai Zhang,Xiaohong Tan,Chong Zhang,Yuling Tang,Yan Teng,Xiao Yang

Nature communications 7:13478 PubMed27876794

2016

VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Miyamoto,Tomohiro Torii,Akito Tanoue,Junji Yamauchi

FASEB journal : official publication of the Federa 30:2860-73 PubMed27127100

2016

In β-actin knockouts, epigenetic reprogramming and rDNA transcription inactivation lead to growth and proliferation defects.

Applications

Unspecified application

Species

Unspecified reactive species

Bader Almuzzaini,Aishe A Sarshad,Aldwin S Rahmanto,Magnus L Hansson,Anne Von Euler,Olle Sangfelt,Neus Visa,Ann-Kristin Östlund Farrants,Piergiorgio Percipalle

Nature protocols 9:2329-40 PubMed25188634

2014

Generation of cerebral organoids from human pluripotent stem cells.

Applications

Unspecified application

Species

Human

Madeline A Lancaster,Juergen A Knoblich

Nature 501:373-9 PubMed23995685

2013

Cerebral organoids model human brain development and microcephaly.

Applications

Unspecified application

Species

Human

Madeline A Lancaster,Magdalena Renner,Carol-Anne Martin,Daniel Wenzel,Louise S Bicknell,Matthew E Hurles,Tessa Homfray,Josef M Penninger,Andrew P Jackson,Juergen A Knoblich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

OI-RD Scanning - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] - BSA and Azide free (AB227574)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise