Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)

This data was developed using ab133638, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling TTF1/Nkx2-1 with purified ab133638 at 1 : 100 (1.39 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)

This data was developed using ab133638, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified ab133638 at 1 : 100 (1.39 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)

This data was developed using ab133638, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling TTF1/Nkx2-1 with purified ab133638 at 1 : 100 (1.39 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)

This data was developed using ab133638, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] (<a href='/en-us/products/primary-antibodies/ttf1-nkx2-1-antibody-epr8190-6-ab133638'>ab133638</a>) at 1/1000 dilution

Lane 1:

Human lung lysate at 15 µg

Lane 2:

Human thyroid lysate at 15 µg

Lane 3:

Rat lung lysate at 15 µg

Lane 4:

Mouse lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 39 kDa

Observed band size: 38-42 kDa

false