Rabbit Recombinant Monoclonal TTF1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--western-blot-img165263.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171743.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174719.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172580.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (AB227575)")
Publications
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- Cell death and differentiation 24:1761-17712017RIP4 inhibits STAT3 signaling to sustain lung adenocarcinoma differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJawahar Kopparam et. al.PubMed 28574510
- Proceedings of the National Academy of Sciences of 113:7614-92016Ablation of Liver X receptors α and β leads to spontaneous peripheral squamous cell lung cancer in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Bing Dai et. al.PubMed 27335465
- Cancer research 74:4676-842014Kinase domain activation of FGFR2 yields high-grade lung adenocarcinoma sensitive to a Pan-FGFR inhibitor in a mouse model of NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy H Tchaicha et. al.PubMed 25035393
- Neuroscience 270:148-572014Prenatal betamethasone does not affect glutamatergic or GABAergic neurogenesis in preterm newborns.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL R Vose et. al.PubMed 24735821
- Nature communications 5:32612014Transdifferentiation of lung adenocarcinoma in mice with Lkb1 deficiency to squamous cell carcinoma.Applications:IHC-PReactive species:MouseXiangkun Han,Fuming Li,Zhaoyuan Fang,Yijun Gao,Fei Li,Rong Fang,Shun Yao,Yihua Sun,Li Li,Wenjing Zhang,Huimin Ma,Qian Xiao,Gaoxiang Ge,Jing Fang,Hongda Wang,Lei Zhang,Kwok-kin Wong,Haiquan Chen,Yingyong Hou,Hongbin JiPubMed 24531128
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Alternative names
NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP
Recommended products
Rabbit Recombinant Monoclonal TTF1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8190-6
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227575 is the carrier-free version of Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
- Biological function summary
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
- Pathways
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
- Associated diseases and disorders
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--western-blot-img165263.jpg "Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)")
Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
This data was developed using Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] (Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638) at 1/1000 dilution
Lane 1: Human lung lysate at 15 µg
Lane 2: Human thyroid lysate at 15 µg
Lane 3: Rat lung lysate at 15 µg
Lane 4: Mouse lung lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 38-42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171743.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
This data was developed using Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638 at 1:100 (1.39 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174719.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
This data was developed using Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling TTF1/Nkx2-1 with purified Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638 at 1:100 (1.39 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-epr8190-6-bsa-and-azide-free-ab227575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172580.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR8190-6] - BSA and Azide free (ab227575)
This data was developed using Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling TTF1/Nkx2-1 with purified Anti-TTF1/Nkx2-1 antibody [EPR8190-6] ab133638 at 1:100 (1.39 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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