Mouse Monoclonal TTF1 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-1.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (AB220211), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-nx21-690-ab220211--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115101.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (AB220211)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (AB220211), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-nx21-690-ab220211--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111261.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (AB220211)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (AB220211), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-nx21-690-ab220211--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118607.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (AB220211)")
Publications
Filter by
- Cellular physiology and biochemistry : internation 44:2138-21462017CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiangmin Zhao et. al.PubMed 29241190
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NKX2-1. The exact immunogen used to generate this antibody is proprietary information. Database link P43699
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).
Alternative names
NKX2A, TITF1, TTF1, NKX2-1, Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP
Recommended products
Mouse Monoclonal TTF1 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NKX2-1. The exact immunogen used to generate this antibody is proprietary information. Database link P43699
- Clone number
- NX2.1/690
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
- Biological function summary
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
- Pathways
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
- Associated diseases and disorders
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-nx21-690-ab220211--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115101.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human lung adenocarcinoma tissue labeling TTF1/Nkx2-1 with ab220211 at 1 μg/mL.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-nx21-690-ab220211--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111261.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human thyroid tissue labeling TTF1/Nkx2-1 with ab220211 at 1 μg/mL.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211), expandable thumbnail](https://content.abcam.com/products/images/ttf1-nkx2-1-antibody-nx21-690-ab220211--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118607.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [NX2.1/690] (ab220211)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat lung tissue labeling TTF1/Nkx2-1 with ab220211 at 1 μg/mL.
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