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Rabbit Recombinant Monoclonal TTF1 antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

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Images

Flow Cytometry (Intracellular) - Anti-TTF1/Nkx2-1 antibody [SP141] (AB227652), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (AB227652), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (AB227652), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (AB227652), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TTF1/Nkx2-1 antibody [SP141] (AB227652), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PICC/IFIHC-Fr
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Primary antibody incubation for 30 minutes at 4°C.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Species

Rat

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Species

Human

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval with 1 mM EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Tested
Tested

Species

Human

Dilution info

1/25

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/20

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity).

Alternative names

Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP, TTF1, TITF1, NKX2A, NKX2-1

Recommended products

Rabbit Recombinant Monoclonal TTF1 antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

Alternative names

Homeobox protein Nkx-2.1, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein, TTF-1, T/EBP, TTF1, TITF1, NKX2A, NKX2-1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP141

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.

Biological function summary

TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.

Pathways

The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.

Associated diseases and disorders

TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.

Product promise

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11 product images

  • Flow Cytometry (Intracellular) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling TTF1/Nkx2-1 with purified ab227652 at 1:170 dilution (1 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat lung tissue sections labeling TTF1/Nkx2-1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse lung tissue sections labeling TTF1/Nkx2-1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid tissue sections labeling TTF1/Nkx2-1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry (Intracellular) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling TTF1/Nkx2-1 with ab227652 at 1/100 dilution (green) compared with a rabbit IgG negative control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling TTF1/Nkx2-1 with ab227652 at 1/100 dilution (1.70 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling TTF1/Nkx2-1 with purified ab227652 at 1:25 (6.8 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Immunohistochemistry (Frozen sections) analysis of rat lung tissue sections labeling TTF1/Nkx2-1 with purified ab227652 at 1:20 (8.5 µg/ml). Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Fixed with 4% paraformaldehyde and permeabilised using 0.2% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. DAPI was used as a Nuclear stain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Formalin-fixed, paraffin-embedded human thyroid tissue stained for TTF1/Nkx2-1 using ab227652 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Formalin-fixed, paraffin-embedded human lung tissue stained for TTF1/Nkx2-1 using ab227652 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [SP141] (ab227652)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TTF1/Nkx2-1 using ab227652 at 1/100 dilution in immunohistochemical analysis.

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