Sheep Polyclonal TTF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TTF2.
View Alternative Names
Transcription termination factor 2, Lodestar homolog, RNA polymerase II termination factor, Transcription release factor 2, F2, HuF2, TTF2
- WB
Unknown
Western blot - Anti-TTF2 antibody (AB28030)
All lanes:
Western blot - Anti-TTF2 antibody (ab28030) at 1/1000 dilution
All lanes:
HeLa nuclear extract
Secondary
All lanes:
rabbit anti-sheep peroxidase linked at 1/10000 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa
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- WB
CiteAb
Western blot - Anti-TTF2 antibody (AB28030)
TTF2 western blot using anti-TTF2 antibody ab28030. Publication image and figure legend from Ramakrishnan, R., Liu, H., et al., 2012, Retrovirology, PubMed 23110726.
ab28030 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab28030 please see the product overview.
SiRNA depletions of protein subunits of CCAPs. TZM-bl cells were transfected with control siRNA or siRNAs against indicated proteins for 72 hours. Cell lysates were prepared, and protein expression was analysed by immunoblots. β-actin was used as a loading control. (A) Panel shows expression of TPR in cells transfected with control or siRNA against TPR, WRNIP1, TTF2 and FBXO11; (B) Top and middle panels show expression level of TTF2 and FBXO11, respectively, in cells transfected with control or siRNA against WRNIP1, TTF2 or FBXO11; (C and D) Panels show expression of WRNIP1 and PPP1R10 in cells transfected with siRNAs against WRNIP1 and PPP1R10, respectively. (E) Graph shows cell viability following transfection of control or PP1R10 siRNAs; the data are the average of three independent experiments with viability of control normalized to 100.
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Reactivity data
Properties and storage information
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Shipped at conditions
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Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TTF2 plays a significant role in the dissociation of the transcription machinery ensuring effective recycling of RNA polymerase II for reuse in new transcription cycles. TTF2 is not a part of a multi-protein complex but works closely with other factors in post-transcriptional regulation. This protein facilitates the resetting of the chromatin environment which is important for the maintenance of gene expression fidelity across cellular processes.
Pathways
TTF2 interacts with several important transcriptional regulation pathways. It plays an integral role in the RNA polymerase II transcription termination pathway ensuring proper end of transcription. Furthermore its functional association with proteins like RNA polymerase II and termination-related cofactors emphasizes its role in efficient transcriptional control and regulatory processes that affect gene expression.
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Publications (1)
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Retrovirology 9:90 PubMed23110726
2012
Applications
WB
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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