Sheep Polyclonal TTF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TTF2.
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- Retrovirology 9:902012Identification of novel CDK9 and Cyclin T1-associated protein complexes (CCAPs) whose siRNA depletion enhances HIV-1 Tat function.Applications:WBReactive species:Unspecified reactive speciesRajesh Ramakrishnan,Hongbing Liu,Hart Donahue,Anna Malovannaya,Jun Qin,Andrew P RicePubMed 23110726
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TTF2. Database link Q9UNY4
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
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Target data
Function
DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
Alternative names
Transcription termination factor 2, Lodestar homolog, RNA polymerase II termination factor, Transcription release factor 2, F2, HuF2, TTF2
Recommended products
Sheep Polyclonal TTF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TTF2.
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TTF2. Database link Q9UNY4
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTF2 also known as transcription termination factor 2 is a protein involved in the removal of RNA polymerase II from chromatin during the process of transcription termination. The protein has a molecular mass of approximately 87 kDa. TTF2 shows expression in various tissues including high levels in the testis and lower levels in the brain and heart. Its activity is essential for ensuring that proper termination signals within the genomic DNA are recognized and that transcription is concluded accurately without inappropriate elongation.
- Biological function summary
TTF2 plays a significant role in the dissociation of the transcription machinery ensuring effective recycling of RNA polymerase II for reuse in new transcription cycles. TTF2 is not a part of a multi-protein complex but works closely with other factors in post-transcriptional regulation. This protein facilitates the resetting of the chromatin environment which is important for the maintenance of gene expression fidelity across cellular processes.
- Pathways
TTF2 interacts with several important transcriptional regulation pathways. It plays an integral role in the RNA polymerase II transcription termination pathway ensuring proper end of transcription. Furthermore its functional association with proteins like RNA polymerase II and termination-related cofactors emphasizes its role in efficient transcriptional control and regulatory processes that affect gene expression.
- Associated diseases and disorders
TTF2 has been implicated in various cancers where transcriptional misregulation is a contributing factor. Changes or anomalies in TTF2 expression or function can disrupt normal transcription termination leading to aberrant gene expression and possibly promoting oncogenesis. Additionally TTF2's interaction with RNA polymerase II highlights its potential involvement in neurodegenerative disorders where precise gene regulation is affected. Understanding TTF2's role in these contexts can benefit the development of targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TTF2 antibody (ab28030)
All lanes: Western blot - Anti-TTF2 antibody (ab28030) at 1/1000 dilution
All lanes: HeLa nuclear extract
SecondaryAll lanes: rabbit anti-sheep peroxidase linked at 1/10000 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-TTF2 antibody (ab28030)
TTF2 western blot using anti-TTF2 antibody ab28030. Publication image and figure legend from Ramakrishnan, R., Liu, H., et al., 2012, Retrovirology, PubMed 23110726.
ab28030 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab28030 please see the product overview.
SiRNA depletions of protein subunits of CCAPs. TZM-bl cells were transfected with control siRNA or siRNAs against indicated proteins for 72 hours. Cell lysates were prepared, and protein expression was analysed by immunoblots. β-actin was used as a loading control. (A) Panel shows expression of TPR in cells transfected with control or siRNA against TPR, WRNIP1, TTF2 and FBXO11; (B) Top and middle panels show expression level of TTF2 and FBXO11, respectively, in cells transfected with control or siRNA against WRNIP1, TTF2 or FBXO11; (C and D) Panels show expression of WRNIP1 and PPP1R10 in cells transfected with siRNAs against WRNIP1 and PPP1R10, respectively. (E) Graph shows cell viability following transfection of control or PP1R10 siRNAs; the data are the average of three independent experiments with viability of control normalized to 100.
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