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AB240241

Anti-TTF2 antibody [EPR12756] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TTF2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Transcription termination factor 2, Lodestar homolog, RNA polymerase II termination factor, Transcription release factor 2, F2, HuF2, TTF2

4 Images
Flow Cytometry (Intracellular) - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TTF2 with ab181116 at 1/60 dilution, followed by Goat anti rabbit IgG (FITC) at 1/150 dilution (pink). Rabbit monoclonal IgG was used as Isotype control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181116).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling TTF2 with ab181116 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181116).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)

Immunofluorescent analysis of acetone-fixed Jurkat cells labeling TTF2 with ab181116 at 1/100 (green) dilution. Goat anti rabbit IgG (Alexa Fluor® 488) was used for secondary antibody staining at a dilution of 1/200. Counter stain : Dapi (blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181116).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] - BSA and Azide free (AB240241)

Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue labeling TTF2 with ab181116 at 1/250. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181116).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12756

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab240241 is the carrier-free version of ab181116.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTF2 also known as transcription termination factor 2 is a protein involved in the removal of RNA polymerase II from chromatin during the process of transcription termination. The protein has a molecular mass of approximately 87 kDa. TTF2 shows expression in various tissues including high levels in the testis and lower levels in the brain and heart. Its activity is essential for ensuring that proper termination signals within the genomic DNA are recognized and that transcription is concluded accurately without inappropriate elongation.
Biological function summary

TTF2 plays a significant role in the dissociation of the transcription machinery ensuring effective recycling of RNA polymerase II for reuse in new transcription cycles. TTF2 is not a part of a multi-protein complex but works closely with other factors in post-transcriptional regulation. This protein facilitates the resetting of the chromatin environment which is important for the maintenance of gene expression fidelity across cellular processes.

Pathways

TTF2 interacts with several important transcriptional regulation pathways. It plays an integral role in the RNA polymerase II transcription termination pathway ensuring proper end of transcription. Furthermore its functional association with proteins like RNA polymerase II and termination-related cofactors emphasizes its role in efficient transcriptional control and regulatory processes that affect gene expression.

TTF2 has been implicated in various cancers where transcriptional misregulation is a contributing factor. Changes or anomalies in TTF2 expression or function can disrupt normal transcription termination leading to aberrant gene expression and possibly promoting oncogenesis. Additionally TTF2's interaction with RNA polymerase II highlights its potential involvement in neurodegenerative disorders where precise gene regulation is affected. Understanding TTF2's role in these contexts can benefit the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
See full target information TTF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 26:5078-5094 PubMed36071546

2022

ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Zhang,Jiangtao Cui,Leiqun Cao,Xiaoting Tian,Yayou Miao,Yikun Wang,Shiyu Qiu,Wanxin Guo,Lifang Ma,Jinjing Xia,Xiao Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com