AB176696

Anti-TTI1 antibody - N-terminal

Name: Anti-TTI1 antibody - N-terminal (ab176696) | Abcam
Brand: Abcam Limited
SKU: AB176696

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(3 Publications)

Rabbit Polyclonal TTI1 antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TTI1 aa 1-50.

View Alternative Names

KIAA0406, SMG10, TTI1, TELO2-interacting protein 1 homolog, Protein SMG10

2 Images

Supplier Data

Immunoprecipitation - Anti-TTI1 antibody - N-terminal (AB176696)

Immunoprecipitation. ab176696 at 0.4 μg/ml staining TTI1 in HeLa whole cell lysate immunoprecipitated using ab176696 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : control IgG.

All lanes:

Immunoprecipitation - Anti-TTI1 antibody - N-terminal (ab176696)

Predicted band size: 122 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-TTI1 antibody - N-terminal (AB176696)

All lanes:

Western blot - Anti-TTI1 antibody - N-terminal (ab176696) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 122 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TTI1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O43156

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTI1 also known as TEL2-interacting protein 1 is a protein weighing approximately 245 kDa. It is expressed widely across various tissues including liver kidney and brain. Mechanically TTI1 operates as an important scaffolding protein that supports the formation of multiprotein complexes specifically those involved in processes of cell growth and proliferation. TTI1 interacts with other proteins to execute these structural roles facilitating precise coordination required for cellular machinery and function.

Biological function summary

TTI1 is a part of the larger complex known as the TTT complex which consists of TTI1 TELO2 and TTI2 proteins. This protein complex stabilizes another set of critical proteins called PIKKs which include mTOR ATM and ATR. These proteins play roles in regulating cell growth DNA damage response and metabolism. By participating in this complex TTI1 plays a role in maintaining cell integrity by interfacing with cellular stress responses and ensuring proper progression and repair of the cell cycle.

Pathways

TTI1 integrates functions within the mTOR signaling pathway and the DNA damage response pathway. In the mTOR pathway TTI1 influences nutrient signaling and energy balance involving cooperation with mTOR which modulates cell metabolism and proliferation. In the DNA damage response pathway the interactions with ATM and ATR are pivotal as they control aspects of DNA repair processes and cellular checkpoints ensuring genomic stability. These pathways illustrate how TTI1 manages to link nutrient signaling with genome maintenance.

TTI1 has associations with cancer and neurodegenerative diseases. In cancer the interactions with mTOR and ATM pathways often result in aberrant signaling leading to uncontrolled cell proliferation. Evidence suggests that these signaling disruptions may contribute to tumorigenesis and treatment resistance. In neurodegenerative diseases disruptions in TTI1-mediated pathways may result in impaired stress responses and cellular repair mechanisms potentially accelerating neuronal cell damage and death. Understanding these relationships highlights the importance of TTI1 in both cellular physiology and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.

See full target information TTI1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 14:1319785 PubMed38098676

2023

Cranberry, but not D-mannose and ibuprofen, prevents against uropathogenic -induced cell damage and cell death in MDCK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jenane Konesan,Jenny Wang,Kate H Moore,Kylie J Mansfield,Lu Liu

Cancer science 114:855-869 PubMed36403197

2022

TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Xian Zhang,Xin Yang,Zhi-Bo Wu,Zhong-Min Liao,Ding-Guo Wang,Shi-Wei Chen,Feng Lu,Yong-Bing Wu,Shu-Qiang Zhu

Frontiers in genetics 10:1060 PubMed31737043

2019

Novel Compound Heterozygous Mutations in TTI2 Cause Syndromic Intellectual Disability in a Chinese Family.

Applications

Unspecified application

Species

Unspecified reactive species

Rongrong Wang,Shirui Han,Hongyan Liu,Amjad Khan,Habulieti Xiaerbati,Xue Yu,Jia Huang,Xue Zhang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TTI1 antibody - N-terminal

Immunoprecipitation - Anti-TTI1 antibody - N-terminal (AB176696)

Western blot - Anti-TTI1 antibody - N-terminal (AB176696)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise