Rabbit Polyclonal TTI2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TTI2 aa 150-300.
View Alternative Names
C8orf41, TTI2, TELO2-interacting protein 2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTI2 antibody (AB237048)
Paraffin-embedded human adrenal gland tissue stained for TTI2 using ab237048 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTI2 antibody (AB237048)
Paraffin-embedded human colon cancer tissue stained for TTI2 using ab237048 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- WB
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Western blot - Anti-TTI2 antibody (AB237048)
All lanes:
Western blot - Anti-TTI2 antibody (ab237048) at 1/500 dilution
Lane 1:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2:
HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3:
PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 4:
Rat kidney tissue lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
false
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-TTI2 antibody (AB237048)
A549 (Human lung carcinoma cell line) cells stained for TTI2 (green) using ab237048 at 1/33 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TTI2 acts as a stabilizing factor within the TTT complex providing structural support required for maturation and stabilization of PIKKs. This complex is important for protein folding and quality control within cells. TTI2 facilitates protein-protein interactions necessary for the assembly of functional PIKK complexes like ATM and mTOR which regulate cellular responses to DNA damage and nutrient signals. Through these functions TTI2 influences cell cycle regulation and growth.
Pathways
TTI2 contributes significantly to the DNA damage response and mTOR signaling pathways. In the DNA damage response pathway its role within the TTT complex supports the activity of ATM protein an important kinase involved in repairing damaged DNA. In the mTOR signaling pathway TTI2 assists in the regulation of metabolism and growth acting in concert with other components of the TTT complex to maintain proper cell function and survival. This interaction connects TTI2 to various proteins like TTI1 and TELO2 reinforcing its importance within these pathways.
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Publications (1)
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Clinical and experimental pharmacology & physiology 52:e70046 PubMed40365957
2025
Applications
Unspecified application
Species
Unspecified reactive species
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