AB187520

Anti-TTK/Mps1 antibody

Name: Anti-TTK/Mps1 antibody (ab187520) | Abcam
Brand: Abcam Limited
SKU: AB187520
Price: 535 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal TTK/Mps1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TTK aa 500 to C-terminus.

View Alternative Names

MPS1, MPS1L1, TTK, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT

1 Images

Supplier Data

Western blot - Anti-TTK/Mps1 antibody (AB187520)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-TTK/Mps1 antibody (ab187520) at 1/1000 dilution

Lane 1:

U-251MG cell lysate at 25 µg

Lane 2:

Mouse testis tissue lysate at 25 µg

Lane 3:

Mouse spleen tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human TTK aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33981

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTK also known as Mps1 is a dual-specificity protein kinase with a molecular mass of approximately 97 kDa. It plays a mechanical role in the regulation of the cell cycle by phosphorylating various substrates required for accurate chromosome alignment and segregation during mitosis. TTK/Mps1 is expressed in many tissues but shows the highest levels in rapidly dividing cells such as in the testis and in tumors. The presence of this protein in proliferative tissues suggests its involvement in cell cycle control mechanisms.

Biological function summary

TTK/Mps1 protein is essential for maintaining genomic stability. It functions as part of the mitotic checkpoint complex (MCC) which prevents cells from prematurely advancing from metaphase to anaphase until all chromosomes are correctly aligned on the mitotic spindle. By doing so TTK/Mps1 ensures proper chromosomal attachment and tension at kinetochores reducing the likelihood of aneuploidy. This regulation highlights its critical role in preventing genetic variation during cell division.

Pathways

TTK/Mps1 is an important component of the spindle assembly checkpoint (SAC) pathway which plays an important role in cell cycle regulation. It works closely with other proteins such as BUBR1 BUB3 and MAD2 to halt cell cycle progression until chromosomal alignment is verified. In addition TTK/Mps1 interacts with the aurora kinase A (AURKA) pathway which is instrumental in centrosome maturation and spindle assembly further emphasizing its role in ensuring accurate mitosis.

Aberrations in TTK/Mps1 activity are associated with cancer and aneuploidy. Overexpression or amplification of TTK/Mps1 is frequently observed in various tumors linking it with cancer progression and poor prognosis. Its interaction with other proteins like p53 a well-known tumor suppressor is significant in understanding its role in oncogenesis. Therapeutic targeting of TTK/Mps1 is being explored to exploit its critical function in rapidly proliferating cancer cells aiming to induce mitotic catastrophe and mitigate tumor growth.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed : 18243099, PubMed : 28441529, PubMed : 29162720). Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint (PubMed : 18243099, PubMed : 29162720). Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore (PubMed : 18243099). Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments (PubMed : 28441529). Phosphorylates KNL1, KNTC1 and autophosphorylates (PubMed : 28441529).

See full target information TTK

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 161:114486 PubMed36906970

2023

Discovery of drug targets and therapeutic agents based on drug repositioning to treat lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Occam Kelly Graves,Woonghee Kim,Mehmet Özcan,Sajda Ashraf,Hasan Turkez,Meng Yuan,Cheng Zhang,Adil Mardinoglu,Xiangyu Li

Cell stem cell 24:285-298.e5 PubMed30639035

2019

Combined Single-Cell Profiling of lncRNAs and Functional Screening Reveals that H19 Is Pivotal for Embryonic Hematopoietic Stem Cell Development.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Jiayue Xu,Linlin Zhang,Siqi Liu,Yanni Ma,Xin Wen,Junkai Hao,Zongcheng Li,Yanli Ni,Xianlong Li,Fan Zhou,Qingqing Li,Fang Wang,Xiaoshuang Wang,Yanmin Si,Pengcheng Zhang,Chen Liu,Marisa Bartolomei,Fuchou Tang,Bing Liu,Jia Yu,Yu Lan

International journal of clinical and experimental 11:4854-4861 PubMed31949560

2018

Protein kinase TTK promotes proliferation and migration and mediates epithelial-mesenchymal transition in human bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feiran Chen,Peikang Wu,Hailong Hu,Dawei Tian,Ning Jiang,Changli Wu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTK/Mps1 antibody

Western blot - Anti-TTK/Mps1 antibody (AB187520)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise