JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB68611

Anti-TTLL1 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Polyclonal TTLL1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TTLL1.

View Alternative Names

C22orf7, TTLL1, Polyglutamylase complex subunit TTLL1, Tubulin polyglutamylase TTLL1, Tubulin polyglutamylase complex subunit 3, Tubulin--tyrosine ligase-like protein 1, PGs3

1 Images
Western blot - Anti-TTLL1 antibody (AB68611)
  • WB

Unknown

Western blot - Anti-TTLL1 antibody (AB68611)

All lanes:

Western blot - Anti-TTLL1 antibody (ab68611) at 1/500 dilution

Lane 1:

TTLL1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 50 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TTLL1.

O95922

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTLL1 also known as Tubulin Tyrosine Ligase Like 1 is an enzyme with a molecular weight of approximately 42 kDa. It adds polyglutamate chains to tubulin modifying microtubule function. TTLL1 predominantly resides in the brain though various other tissues also express it. This protein plays a mechanical role in maintaining proper microtubule dynamics by post-translational modification of tubulins.
Biological function summary

TTLL1 contributes to the stability and function of neuronal microtubules. It does not function as part of a complex but interacts directly with tubulin subunits. By modifying the microtubules TTLL1 affects cellular processes reliant on cytoskeletal function such as cell division intracellular transport and maintenance of cell shape. This modification often results in regulation and targeting of microtubules to specific cellular locations and functions.

Pathways

TTLL1 participates in cytoskeleton-associated processes influencing the regulation of microtubule-associated pathways. It relates closely to the tubulin code hypothesis which proposes that tubulin post-translational modifications tag microtubules for interaction with microtubule-associated proteins. TTLL1's actions show a relationship with proteins like kinesins and dyneins essential for intracellular transport mechanisms and also influence cell structural integrity through these pathways.

TTLL1 has associations with neurodegenerative diseases worrying scientists in this field. Alterations in its function are linked to cerebellar ataxia a condition that affects movement coordination. Through such a disorder TTLL1 shows connections to other proteins like ataxin suggesting cooperative roles in neuronal stability and function. Researchers also study TTLL1 in the context of cancers as its dysregulation may lead to improper cell division and tumorigenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalytic subunit of a polyglutamylase complex which modifies tubulin, generating side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin (PubMed : 34782749). Probably involved in the side-chain elongation step of the polyglutamylation reaction rather than the initiation step. Modifies both alpha- and beta-tubulins with a preference for the alpha-tail. Unlike most polyglutamylases of the tubulin--tyrosine ligase family, only displays a catalytic activity when in complex with other proteins as it is most likely lacking domains important for autonomous activity. Part of the neuronal tubulin polyglutamylase complex. Mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility. Involved in respiratory motile cilia function through the regulation of beating asymmetry. Essential for sperm flagella biogenesis, motility and male fertility. Involved in KLF4 glutamylation which impedes its ubiquitination, thereby leading to somatic cell reprogramming, pluripotency maintenance and embryogenesis.
See full target information TTLL1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB307678

Anti-VPS54 antibody [EPR28123-81]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-VPS54 antibody [EPR28123-81] (AB307678)

  • 0
  • 0
Primary Antibodies

AB270035

Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (AB270035)

  • 0
  • 0
Primary Antibodies

AB109194

Anti-SOX18 antibody [EPR2837(2)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-SOX18 antibody [EPR2837(2)] (AB109194)

  • 0
  • 0
Primary Antibodies

AB67785

Anti-TRIM31 antibody

Western blot - Anti-TRIM31 antibody (AB67785)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com