AB154086

Anti-TTLL12 antibody

Name: Anti-TTLL12 antibody (ab154086) | Abcam
Brand: Abcam Limited
SKU: AB154086
Price: 495 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal TTLL12 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TTLL12 aa 400 to C-terminus.

View Alternative Names

KIAA0153, TTLL12, Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTLL12 antibody (AB154086)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTLL12 antibody (AB154086)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue labeling TTLL12 with ab154086 at 1/500 dilution.

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Western blot - Anti-TTLL12 antibody (AB154086)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TTLL12 antibody (ab154086) at 1/3000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 74 kDa

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Western blot - Anti-TTLL12 antibody (AB154086)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TTLL12 antibody (ab154086) at 1/5000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TTLL12 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14166

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTLL12 also known as Tubulin Tyrosine Ligase Like 12 is an enzyme that belongs to the tubulin tyrosine ligase-like (TTLL) family. This protein functions mechanically by catalyzing the polyglutamylation of target proteins especially tubulin impacting microtubule stability and interaction. TTLL12 has a mass of approximately 60 kDa and is predominantly expressed in the brain and testis although its expression is noted in other tissues as well. The involvement of TTLL12 in these tissues suggests its significance in specialized cellular processes.

Biological function summary

TTLL12 influences neuronal and cellular functions through its capacity to alter the microtubule network. It participates in the polyglutamylation activity that regulates microtubule dynamics synaptic stability and intracellular transport playing critical roles in neurodevelopment and cellular organization. Although TTLL12 itself does not commonly form large complexes its enzymatic activity impacts other microtubule-modifying complexes indirectly affecting cellular architecture and signaling pathways.

Pathways

TTLL12 participates notably in the microtubule-network pathway and the regulation of cytoskeleton organization. Its activity modulates the polymerization and depolymerization dynamics of microtubules impacting cell cycle and neuronal signaling. TTLL12's function is related to other tubulin-modifying enzymes such as TTLL1 and TTLL5 which share a role in tubulin modification thereby affecting cellular processes like mitosis and intracellular trafficking.

TTLL12's aberrant activity or expression has connections to neurodegenerative diseases and male infertility. For example its dysfunction in the brain is associated with tauopathies where improper microtubule regulation contributes to disease pathology. Additionally TTLL12's function relates to proteins like tau and other microtubule-associated proteins (MAPs) which play roles in maintaining neuronal structure and function. Understanding its connections to these conditions may shed light on potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues (PubMed : 20162578, PubMed : 23251473). Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3) (PubMed : 23251473). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability (PubMed : 20162578, PubMed : 23251473). During RNA virus-mediated infection, acts as a negative regulator of the RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE (PubMed : 28011935).

See full target information TTLL12

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40461158

2025

Tumor-intrinsic TTLL12 drives resistance to cancer immunotherapy via modulating myeloid-derived suppressor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Wen Chen,Yi-Kan Cheng,Pei-Si Li,Xiao-Jian Wu,Xin-Xin Huang,Jia-Hui Long,Zhizhong Xiong,Chong Chen,Shu-Biao Ye,Ping Lan

Nature communications 13:2760 PubMed35589749

2022

Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Odeta Meçe,Diede Houbaert,Maria-Livia Sassano,Tania Durré,Hannelore Maes,Marco Schaaf,Sanket More,Maarten Ganne,Melissa García-Caballero,Mila Borri,Jelle Verhoeven,Madhur Agrawal,Kathryn Jacobs,Gabriele Bergers,Silvia Blacher,Bart Ghesquière,Mieke Dewerchin,Johan V Swinnen,Stefan Vinckier,María S Soengas,Peter Carmeliet,Agnès Noël,Patrizia Agostinis

Oncology letters 20:340 PubMed33123251

2020

Effect of TTLL12 on tubulin tyrosine nitration as a novel target for screening anticancer drugs .

Applications

Unspecified application

Species

Unspecified reactive species

Lingli Xue,Yan Zeng,Chuan Fang,Wei Cheng,Yadong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TTLL12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTLL12 antibody (AB154086)

Western blot - Anti-TTLL12 antibody (AB154086)

Western blot - Anti-TTLL12 antibody (AB154086)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise