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AB155801

Anti-TTLL4 antibody

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(2 Publications)

Rabbit Polyclonal TTLL4 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TTLL4 aa 550-800.

View Alternative Names

KIAA0173, TTLL4, Tubulin monoglutamylase TTLL4, Protein monoglutamylase TTLL4, Tubulin--tyrosine ligase-like protein 4

1 Images
Western blot - Anti-TTLL4 antibody (AB155801)
  • WB

Unknown

Western blot - Anti-TTLL4 antibody (AB155801)

5% SDS PAGE

All lanes:

Western blot - Anti-TTLL4 antibody (ab155801) at 1/500 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

PC3 whole cell lysate at 30 µg

Predicted band size: 133 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TTLL4 aa 550-800. The exact immunogen used to generate this antibody is proprietary information.

Q14679

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Primary Antibodies

AB6046

Anti-beta Tubulin antibody - Loading Control

BOND RX™ Validated

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Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTLL4 also known as Tubulin Tyrosine Ligase-Like Family Member 4 is an enzyme that catalyzes the polyglutamylation of proteins such as tubulin. It is part of the TTLL family of enzymes which add glutamate residues to substrate proteins. TTLL4 has a mass of approximately 141 kDa. This enzyme is mostly found in the brain and testis with higher expression levels in neuronal tissues. TTLL4 participates in the post-translational modification of microtubules impacting their dynamics and stability.
Biological function summary

This enzyme functions in the regulation of microtubule-associated processes. TTLL4 is not believed to be part of a known complex but its activity affects microtubule connections with cellular structures. Polyglutamylation impacts the interaction of microtubules with other proteins influencing cell division intracellular transport and ciliary movement. By modifying tubulin TTLL4 indirectly influences important cell functions through these microtubule-associated processes.

Pathways

TTLL4's activity connects to the regulation of the cytoskeleton and cilia formation. It plays a role in the proper functioning of neuronal cells by modulating microtubule stability through the addition of glutamate chains. TTLL4 works alongside proteins such as TPX2 involved in microtubule nucleation and others participating in cell cycle regulation. This enzyme also impacts pathways related to the stabilization of the axonal cytoskeleton critical for nerve cell function and signaling.

Alteration in TTLL4's function links to neurodegenerative disorders and ciliopathies. Aberrant polyglutamylation may contribute to disorders like Alzheimer's disease by affecting tubulin stability and intracellular transport in association with proteins like tau. Additionally TTLL4 dysfunction may lead to faulty cilia formation and motility central to disorders like retinitis pigmentosa. Understanding its interaction with proteins in these diseases provides insight into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Monoglutamylase which modifies both tubulin and non-tubulin proteins, adding a single glutamate on the gamma-carboxyl group of specific glutamate residues of target proteins. Involved in the side-chain initiation step of the polyglutamylation reaction but not in the elongation step. Preferentially modifies beta-tail tubulin over the alpha-tubulin. Monoglutamylates nucleosome assembly proteins NAP1L1 and NAP1L4. Monoglutamylates nucleotidyltransferase CGAS, leading to inhibition of CGAS catalytic activity, thereby preventing antiviral defense function. Involved in KLF4 glutamylation which impedes its ubiquitination, thereby leading to somatic cell reprogramming, pluripotency maintenance and embryogenesis.
See full target information TTLL4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7699 PubMed40825974

2025

Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Liuwei Huang,Yanting Shen,Xiaoling Pan,Jiaqi Li,Caizhen Li,Lixin Ruan,Sitan He,Lanlan Huang,Kangyi Liu,Xin Zhao,Jian Geng,Jie Guo,Fan Fan Hou,Jun Wang

Journal of cellular and molecular medicine 24:8379-8390 PubMed32639656

2020

P-cresyl sulfate causes mitochondrial hyperfusion in H9C2 cardiomyoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Tien-Hung Huang,Hon-Kan Yip,Cheuk-Kwan Sun,Yi-Ling Chen,Chih-Chao Yang,Fan-Yen Lee
View all publications
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Product promise

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