AB187697

Anti-TTLL5 antibody

Name: Anti-TTLL5 antibody (ab187697) | Abcam
Brand: Abcam Limited
SKU: AB187697

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal TTLL5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TTLL5 aa 1200 to C-terminus.

View Alternative Names

KIAA0998, STAMP, TTLL5, Tubulin polyglutamylase TTLL5, SRC1 and TIF2-associated modulatory protein, Tubulin--tyrosine ligase-like protein 5, STAMP protein

2 Images

Supplier Data

Immunoprecipitation - Anti-TTLL5 antibody (AB187697)

Detection of TTLL5 by Western Blot of Immunprecipitate.

Lane 1 : ab187697 1μg/ml labeling TTLL5 in HeLa whole cell lysate immunoprecipitated using ab187697 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-TTLL5 antibody (ab187697)

Predicted band size: 144 kDa

false

Supplier Data

Western blot - Anti-TTLL5 antibody (AB187697)

All lanes:

Western blot - Anti-TTLL5 antibody (ab187697) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 144 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TTLL5 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6EMB2

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTLL5 or Tubulin Tyrosine Ligase Like 5 acts as an enzyme that adds glutamates onto tubulin a process known as polyglutamylation. It has an approximate mass of 121 kDa. This protein is expressed in various tissues with higher levels observed in the retinal photoreceptor cells and testes. TTLL5 plays a role in the post-translational modification of proteins which is essential for the functioning of the microtubule network.

Biological function summary

TTLL5 contributes to cellular processes by modifying tubulin a critical component of the cytoskeleton. In its role it performs polyglutamylation affecting microtubule stability and interactions with microtubule-associated proteins (MAPs) and motor proteins. TTLL5 functions as a part of a larger group of enzymes similar to other members of the tubulin tyrosine ligase-like family which regulates the different aspects of microtubule dynamics.

Pathways

TTLL5 is important in the MAPK signaling pathway and axon guidance. In these pathways TTLL5 affects cellular morphology and signaling by modifying tubulin structures which may regulate the activity of proteins like MAP2 and tau impacting cell transport and division. Its role in these pathways highlights the significance of microtubule modifications in both cellular signaling and structural integrity.

Mutations or dysregulation of TTLL5 have been linked to retinal degeneration and ciliary dysfunction disorders. In retinal degeneration TTLL5 defects affect photoreceptor cell functions due to improper protein modification. The protein’s impact on the disease connects through interaction with other microtubule-related proteins which are also critical for maintaining retinal cell structure and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Polyglutamylase which modifies tubulin, generating polyglutamate side chains on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Preferentially mediates ATP-dependent initiation step of the polyglutamylation reaction over the elongation step. Preferentially modifies the alpha-tubulin tail over a beta-tail (By similarity). Required for CCSAP localization to both polyglutamylated spindle and cilia microtubules (PubMed : 22493317). Increases the effects of transcriptional coactivator NCOA2/TIF2 in glucocorticoid receptor-mediated repression and induction and in androgen receptor-mediated induction (PubMed : 17116691).

See full target information TTLL5

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70245 PubMed39648156

2024

KLF7 Promotes Hepatocellular Carcinoma Progression Through Regulating SLC1A5-Mediated Tryptophan Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Chai,Anhong Zhang,Yang Liu,Xi Zhang,Pengzhou Kong,Zhuowei Zhang,Yarong Guo

Cell discovery 8:77 PubMed35945223

2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Shupei Wei,Qin Zhao,Ke Zheng,Peiying Liu,Nannan Sha,Yingzi Li,Chunmin Ma,Jingjie Li,Lingang Zhuo,Guanxin Liu,Wenhua Liang,Yuhui Jiang,Tao Chen,Nanshan Zhong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTLL5 antibody

Immunoprecipitation - Anti-TTLL5 antibody (AB187697)

Western blot - Anti-TTLL5 antibody (AB187697)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise