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AB155323

Anti-TTPA/TPP1 antibody

4

(3 Reviews)

|

(1 Publication)

Rabbit Polyclonal TTPA/TPP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TTPA aa 200 to C-terminus.

View Alternative Names

TPP1, TTPA, Alpha-tocopherol transfer protein, Alpha-TTP

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTPA/TPP1 antibody (AB155323)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTPA/TPP1 antibody (AB155323)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling TTPA/TPP1 with ab155323 at 1/500 dilution

Immunocytochemistry/ Immunofluorescence - Anti-TTPA/TPP1 antibody (AB155323)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TTPA/TPP1 antibody (AB155323)

Immunofluorescence analysis of methanol-fixed MCF7 cells labeling TTPA/TPP1 using ab155323 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-TTPA/TPP1 antibody (AB155323)
  • WB

Unknown

Western blot - Anti-TTPA/TPP1 antibody (AB155323)

12% SDS PAGE

All lanes:

Western blot - Anti-TTPA/TPP1 antibody (ab155323) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Western blot - Anti-TTPA/TPP1 antibody (AB155323)
  • WB

Supplier Data

Western blot - Anti-TTPA/TPP1 antibody (AB155323)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-TTPA/TPP1 antibody (ab155323) at 1/500 dilution

All lanes:

Rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Western blot - Anti-TTPA/TPP1 antibody (AB155323)
  • WB

Supplier Data

Western blot - Anti-TTPA/TPP1 antibody (AB155323)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-TTPA/TPP1 antibody (ab155323) at 1/500 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TTPA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49638

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTPA also known as tocopherol (alpha) transfer protein and TPP1 is a protein with a mass of approximately 32 kDa. The protein binds to alpha-tocopherol a type of vitamin E and facilitates its transfer to cellular membranes. TTPA is mainly expressed in the liver but also found in smaller amounts in the brain lungs and other tissues. The protein ensures the efficient distribution of vitamin E throughout the body playing a significant role in maintaining cellular health.
Biological function summary

In many organisms TTPA acts as an important transporter of vitamin E helping protect cells against oxidative damage by delivering it to sites where it acts as an antioxidant. While not formally part of a recognized complex the protein interacts with cellular membranes as it transfers alpha-tocopherol. The function supports cell protection and injury prevention by ensuring an adequate supply of this essential vitamin.

Pathways

Vitamin E's movement within the body is directly influenced by TTPA's activity placing the protein within the tocopherol transport and metabolism pathways. The interaction with other antioxidant systems like the glutathione pathway highlights TTPA's importance in cellular redox homeostasis. TTPA-related proteins such as vitamin E-binding proteins modulate this transport and influence overall oxidative stress responses.

Mutations in the TTPA gene can lead to a rare neurodegenerative disorder known as ataxia with vitamin E deficiency (AVED). This condition highlights the critical need for functional TTPA in neurological health. Additionally research has linked TTPA to liver-related conditions where an association is found with lower levels of vitamin E and liver damage emphasizing its role as a protector of liver tissues. Understanding these connections helps target TTPA-related pathways and proteins such as APOB for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds alpha-tocopherol, enhances its transfer between separate membranes, and stimulates its release from liver cells (PubMed : 7887897). Binds both phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate; the resulting conformation change is important for the release of the bound alpha-tocopherol (By similarity).
See full target information TTPA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 21:564 PubMed33850536

2021

C-C motif chemokine ligand 2/C-C receptor 2 is associated with glioma recurrence and poor survival.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuan Yang,Junpeng Zhang,Xin Zhang,Lifeng Miao,Wei Zhang,Zheng Jiang,Wei Zhou
View all publications
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Product promise

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