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Rabbit Polyclonal TTPA/TPP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TTPA aa 200 to C-terminus.


Images

Western blot - Anti-TTPA/TPP1 antibody (AB155323), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TTPA/TPP1 antibody (AB155323), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTPA/TPP1 antibody (AB155323), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human TTPA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49638

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Target data

Function

Binds alpha-tocopherol, enhances its transfer between separate membranes, and stimulates its release from liver cells (PubMed:7887897). Binds both phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate; the resulting conformation change is important for the release of the bound alpha-tocopherol (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal TTPA/TPP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TTPA aa 200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human TTPA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P49638
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TTPA also known as tocopherol (alpha) transfer protein and TPP1 is a protein with a mass of approximately 32 kDa. The protein binds to alpha-tocopherol a type of vitamin E and facilitates its transfer to cellular membranes. TTPA is mainly expressed in the liver but also found in smaller amounts in the brain lungs and other tissues. The protein ensures the efficient distribution of vitamin E throughout the body playing a significant role in maintaining cellular health.

Biological function summary

In many organisms TTPA acts as an important transporter of vitamin E helping protect cells against oxidative damage by delivering it to sites where it acts as an antioxidant. While not formally part of a recognized complex the protein interacts with cellular membranes as it transfers alpha-tocopherol. The function supports cell protection and injury prevention by ensuring an adequate supply of this essential vitamin.

Pathways

Vitamin E's movement within the body is directly influenced by TTPA's activity placing the protein within the tocopherol transport and metabolism pathways. The interaction with other antioxidant systems like the glutathione pathway highlights TTPA's importance in cellular redox homeostasis. TTPA-related proteins such as vitamin E-binding proteins modulate this transport and influence overall oxidative stress responses.

Associated diseases and disorders

Mutations in the TTPA gene can lead to a rare neurodegenerative disorder known as ataxia with vitamin E deficiency (AVED). This condition highlights the critical need for functional TTPA in neurological health. Additionally research has linked TTPA to liver-related conditions where an association is found with lower levels of vitamin E and liver damage emphasizing its role as a protector of liver tissues. Understanding these connections helps target TTPA-related pathways and proteins such as APOB for potential therapeutic interventions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-TTPA/TPP1 antibody (ab155323), expandable thumbnail

    Western blot - Anti-TTPA/TPP1 antibody (ab155323)

    12% SDS PAGE

    All lanes: Western blot - Anti-TTPA/TPP1 antibody (ab155323) at 1/1000 dilution

    All lanes: IMR32 whole cell lysate at 30 µg

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TTPA/TPP1 antibody (ab155323), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TTPA/TPP1 antibody (ab155323)

    Immunofluorescence analysis of methanol-fixed MCF7 cells labeling TTPA/TPP1 using ab155323 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTPA/TPP1 antibody (ab155323), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTPA/TPP1 antibody (ab155323)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling TTPA/TPP1 with ab155323 at 1/500 dilution

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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