Rabbit Polyclonal TTYH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein tweety homolog 1 aa 250-400.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Protein tweety homolog 1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H313
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Cynomolgus monkey | Dilution info - | Notes - |
Target data
Function
Calcium-independent, swelling-dependent volume-regulated anion channel (VRAC-swell) which plays a pivotal role in the process of regulatory volume decrease (RVD) in the brain through the efflux of anions like chloride and organic osmolytes like glutamate. Isoform 3. Ca(2+)-independent, swelling-activated chloride channel, possibly involved in regulation of cell volume.
Alternative names
Protein tweety homolog 1, hTTY1, Volume-regulated anion channel subunit TTYH1, TTYH1
Recommended products
Rabbit Polyclonal TTYH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein tweety homolog 1 aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Protein tweety homolog 1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H313
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTYH1 also known as Tweety homolog 1 is a transmembrane protein that belongs to the Tweety protein family. It has a mass of approximately 60 kDa. TTYH1 functions as a chloride channel and shows expression notably in the central nervous system particularly in the brain. Researchers observe its presence in several cell types including neurons and glial cells. Its expression pattern suggests a role linked to neural development and function.
- Biological function summary
TTYH1 participates in the regulation of ion transport across cellular membranes. This function plays a significant role in maintaining cellular homeostasis and electrical excitability. TTYH1 supports chloride ion movement which influences cell volume regulation and neural signal transduction. TTYH1 might associate with other channel proteins and complexes that contribute to membrane potential and cell signaling processes impacting neurological processes.
- Pathways
TTYH1 contributes to ion homeostasis and synaptic transmission processes within neural pathways. It interacts notably with proteins involved in the chloride ion transport pathway essential for maintaining proper neuronal activity. One of these other proteins is CLCN2 a chloride channel protein. TTYH1's function aligns with its role in maintaining the ion balance necessary for synaptic activity and effective neurotransmission.
- Associated diseases and disorders
Aberrant TTYH1 activity potentially links to neurological disorders including epilepsy and glioma. The abnormal function or expression of TTYH1 may impact the balance of ions within neurons which carries implications for neural excitability and seizure susceptibility in epilepsy. Additionally its expression in glioma suggests a role in the progression or pathology of tumor growth in the central nervous system. TTYH1's link with such conditions draws attention to its potential interactions with proteins like connexin43 which is involved in cell-to-cell communication and could further influence disease dynamics.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TTYH1 antibody (ab204046)
All lanes: Western blot - Anti-TTYH1 antibody (ab204046) at 0.4 µg/mL
All lanes: Human cerebral cortex tissue lysate
Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTYH1 antibody (ab204046)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling TTYH1 in the neuropil with ab204046 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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