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Rabbit Recombinant Monoclonal TBA3 antibody. Carrier free. Suitable for WB and reacts with Saccharomyces cerevisiae samples.

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Images

Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (AB250803), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Saccharomyces cerevisiae
Tested

Tested
Tested

Species
Saccharomyces cerevisiae
Dilution info
-
Notes

-

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

YML124C, YM7056.02C, TUB3, Tubulin alpha-3 chain

Recommended products

Rabbit Recombinant Monoclonal TBA3 antibody. Carrier free. Suitable for WB and reacts with Saccharomyces cerevisiae samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13800
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250803 is the carrier-free version of Anti-TUB3 antibody [EPR13800] ab184971.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TUB3 also known as Tubulin Alpha 3 is a component of microtubules which are cylindrical structures within cells. It has a mass of approximately 50 kDa. TUB3 polymerizes with beta-tubulin to form the tubulin heterodimer which is the building block of microtubules. This protein is expressed in a variety of tissues including the brain and muscles which highlights its importance in these locations.

Biological function summary

TUB3 plays a role in the stabilization of microtubule structures. As a part of the tubulin family it interacts within a larger complex to support cellular dynamics such as shape motility and division. It is essential for processes like intracellular transport where vesicles and organelles rely on microtubules to journey through the cytoplasm. TUB3 also contributes to the proper operation of the mitotic spindle during cell division ensuring accurate chromosome segregation.

Pathways

Its involvement includes the cytoskeleton organization pathway and the mitosis pathway. During cell cycling TUB3 interacts with key proteins like kinesin and dynein which are responsible for motor activities along microtubules. This interaction supports mitotic spindle assembly an important process during the cell replication cycle. TUB3 facilitates various cellular mechanisms tied to structural maintenance and functional operations.

Associated diseases and disorders

TUB3 has relevancy to neurodegenerative conditions and cancer. Mutations or dysregulation of TUB3 link to Alzheimer's disease where it might affect neuronal transport and stability. In cancer TUB3 expression levels can influence cell proliferation rates. The protein's interplay with many cancer-related kinases such as GSK-3 portrays its potential impact on tumor growth and advancement. Understanding and targeting TUB3 could offer new angles for therapeutic development in these areas.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (ab250803), expandable thumbnail

    Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (ab250803)

    This data was developed using Anti-TUB3 antibody [EPR13800] ab184971, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TUB3 antibody [EPR13800] (Anti-TUB3 antibody [EPR13800] ab184971) at 1/50000 dilution

    All lanes: Saccharomyces cerevisiae extract at 10 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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