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AB250803

Anti-TUB3 antibody [EPR13800] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TBA3 antibody. Carrier free. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

View Alternative Names

YML124C, YM7056.02C, TUB3, Tubulin alpha-3 chain

1 Images
Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (AB250803)
  • WB

Supplier Data

Western blot - Anti-TUB3 antibody [EPR13800] - BSA and Azide free (AB250803)

This data was developed using ab184971, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TUB3 antibody [EPR13800] (<a href='/en-us/products/primary-antibodies/tub3-antibody-epr13800-ab184971'>ab184971</a>) at 1/50000 dilution

All lanes:

Saccharomyces cerevisiae extract at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13800

Isotype

IgG

Carrier free

Yes

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250803 is the carrier-free version of ab184971.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUB3 also known as Tubulin Alpha 3 is a component of microtubules which are cylindrical structures within cells. It has a mass of approximately 50 kDa. TUB3 polymerizes with beta-tubulin to form the tubulin heterodimer which is the building block of microtubules. This protein is expressed in a variety of tissues including the brain and muscles which highlights its importance in these locations.
Biological function summary

TUB3 plays a role in the stabilization of microtubule structures. As a part of the tubulin family it interacts within a larger complex to support cellular dynamics such as shape motility and division. It is essential for processes like intracellular transport where vesicles and organelles rely on microtubules to journey through the cytoplasm. TUB3 also contributes to the proper operation of the mitotic spindle during cell division ensuring accurate chromosome segregation.

Pathways

Its involvement includes the cytoskeleton organization pathway and the mitosis pathway. During cell cycling TUB3 interacts with key proteins like kinesin and dynein which are responsible for motor activities along microtubules. This interaction supports mitotic spindle assembly an important process during the cell replication cycle. TUB3 facilitates various cellular mechanisms tied to structural maintenance and functional operations.

TUB3 has relevancy to neurodegenerative conditions and cancer. Mutations or dysregulation of TUB3 link to Alzheimer's disease where it might affect neuronal transport and stability. In cancer TUB3 expression levels can influence cell proliferation rates. The protein's interplay with many cancer-related kinases such as GSK-3 portrays its potential impact on tumor growth and advancement. Understanding and targeting TUB3 could offer new angles for therapeutic development in these areas.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
See full target information TUB3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com