AB95966

Anti-TUBA1A antibody - Loading Control

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(2 Publications)

Rabbit Polyclonal TBA1A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA1A aa 1 to C-terminus.

View Alternative Names

TUBA3, Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain

7 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TUBA1A antibody - Loading Control (AB95966)

Immunofluorescence analysis of 4% paraformaldehyde fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TUBA1A at cytoskeleton with ab95966 at 1/500 dilution. Blue : Hoechst 33342 staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TUBA1A antibody - Loading Control (AB95966)

Immunofluorescence analysis of 4% paraformaldehyde fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells lalbeling TUBA1A at cytoskeleton with ab95966 at 1/500 dilution. Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TUBA1A antibody - Loading Control (ab95966) at 1/10000 dilution

Lane 1:

293T whole cell extracts at 15 µg

Lane 2:

293T whole cell extracts at 5 µg

Lane 3:

293T whole cell extracts at 2 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA1A antibody - Loading Control (ab95966) at 1/10000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 4:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 6:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Lane 7:

H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

Lane 8:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 10:

NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg

Lane 11:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 12:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA1A antibody - Loading Control (ab95966) at 1/10000 dilution

All lanes:

Rat brain tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA1A antibody - Loading Control (ab95966) at 1/10000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 2:

Mouse brain tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA1A antibody - Loading Control (ab95966) at 1/10000 dilution

All lanes:

Drosophila lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Drosophila melanogaster

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TUBA1A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q71U36

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBA1A also known as alpha-tubulin 1A or Tubulin Alpha-1A Chain is a protein with a molecular mass of about 50 kDa. It is an important subunit of tubulin which polymerizes to form microtubules essential components of the cytoskeleton. TUBA1A is expressed in a wide range of tissues but shows higher levels in the central nervous system as tubulin forms the structural framework necessary for neuron function and cellular integrity.

Biological function summary

The TUBA1A protein functions in the assembly and disassembly of microtubules participating as part of the heterodimeric tubulin complex along with beta-tubulin. This structure allows microtubules to contribute to intracellular transport cell motility and chromosome segregation during cell division. The dynamic instability of microtubules facilitated by tubulin proteins like TUBA1A is important for the adaptive cellular functions.

Pathways

TUBA1A plays a role in the microtubule-associated processes being involved in the MAPK/ERK pathway and the mTOR signaling pathway. These pathways are important for cell growth differentiation and survival. TUBA1A's interaction with other microtubule-associated proteins regulates these signaling events and it also associates with proteins such as MAP2 or tau influencing the organization of microtubule networks.

Mutations in the TUBA1A gene relate to conditions like lissencephaly and microlissencephaly which affect brain development and lead to neurological deficits. In these disorders TUBA1A mutations disrupt normal microtubule function often influencing other proteins such as LIS1 which is critical for proper neuronal migration. Understanding TUBA1A's role can help in diagnosing and exploring therapeutics for such neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA1A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 12:33 PubMed39468645

2024

CYP19A1 regulates chemoresistance in colorectal cancer through modulation of estrogen biosynthesis and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Qiang Ji,Ning Cao,Guijie Ge,Xiaomin Li,Xiangdong Liu,Yanqi Mi

Cell chemical biology 23:517-28 PubMed27105284

2016

p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nam-Gu Her,Julia I Toth,Chen-Ting Ma,Yang Wei,Khatereh Motamedchaboki,Eduard Sergienko,Matthew D Petroski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUBA1A antibody - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-TUBA1A antibody - Loading Control (AB95966)

Immunocytochemistry/ Immunofluorescence - Anti-TUBA1A antibody - Loading Control (AB95966)

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

Western blot - Anti-TUBA1A antibody - Loading Control (AB95966)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise