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AB305163

Anti-TUBA1B antibody [EPR24627-4] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

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Sandwich ELISA - Anti-TUBA1B antibody [EPR24627-4] - BSA and Azide free (Detector) (AB305163)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TUBA1B antibody [EPR24627-4] - BSA and Azide free (Detector) (AB305163)

Sandwich ELISA of ab305095 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24627-4

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBA1B also known as tubulin alpha-1B chain is a subunit of microtubules that plays a structural role in the cellular cytoskeleton. It has a molecular mass of about 50 kDa. TUBA1B distributes widely across various tissues with elevated expression in neuronal cells. It characterizes by its involvement in the assembly and stabilization of microtubules which are essential for maintaining cell shape promoting intracellular transport and facilitating chromosome segregation during cell division.
Biological function summary

TUBA1B contributes significantly to the formation and function of the cytoskeletal microtubule network. It functions as part of the heterodimeric complex pairing with beta-tubulin to form the building blocks of microtubules. These structures are central to diverse cellular activities such as intracellular trafficking axonal transport in neurons and the migration and division of cells. In this implicit role TUBA1B influences the dynamics and stability of microtubule polymers impacting the cell’s ability to respond to mechanical and environmental stimuli.

Pathways

TUBA1B is integral to the mitotic spindle assembly pathway and neuronal signaling. In the context of these pathways TUBA1B's interactions with proteins like dynein and kinesin facilitate its role in intracellular transport and spindle orientation during cell division. TUBA1B also participates in neuronal development pathways where its association with tau and other microtubule-associated proteins contributes to the formation of neuronal axons and dendrites.

Alterations in TUBA1B have been linked to certain neurodevelopmental disorders and cancers such as lissencephaly and glioblastoma. In lissencephaly mutations in TUBA1B disrupt proper neuronal migration contributing to the abnormal brain development associated with the disorder. In glioblastoma TUBA1B's aberrant expression impacts microtubule dynamics aiding in cancer cell proliferation and resistance to therapies. These pathological conditions often involve interactions with other proteins including those found in the microtubule-associated protein family such as MAP2 and MAPT which further influence disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed : 34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871).
See full target information TUBA1B acetyl K40
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Product promise

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For full details, please see our Terms & Conditions

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