Rabbit Polyclonal TUBA1C antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA1C aa 1-200.
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Publications
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- BMC cancer 23:7162023TUBA1C is a potential new prognostic biomarker and promotes bladder urothelial carcinoma progression by regulating the cell cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Jiang,Chao Zhu,Haoxuan Huang,Gaomin Huang,Bin Fu,Xiaoqing XiPubMed 37528357
- Frontiers in behavioral neuroscience 16:8679582022Predictors of prodromal Parkinson's disease in young adult rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah A Lechner,Jacob M Welsch,Natalie K Pahapill,Taylor A R Kaldenberg,Amy Regenbaum,Cynthia A Kelm-NelsonPubMed 36172466
- Cancer cell international 21:1442021Prognostic biomarker TUBA1C is correlated to immune cell infiltration in the tumor microenvironment of lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Bian,Miaosen Zheng,Daishan Jiang,Jian Liu,Hui Sun,Xiaoli Li,Lei Liu,Jianguo Zhang,Yifei LiuPubMed 33653340
- Frontiers in oncology 10:492020Upregulated Expression of TUBA1C Predicts Poor Prognosis and Promotes Oncogenesis in Pancreatic Ductal Adenocarcinoma via Regulating the Cell Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMugahed Abdullah Hasan Albahde,Piao Zhang,Qiuqiang Zhang,Guoqi Li,Weilin WangPubMed 32117719
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TUBA1C aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BQE3
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chinese hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chinese hamster | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chinese hamster | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBA6, TUBA1C, Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain
Recommended products
Rabbit Polyclonal TUBA1C antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA1C aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TUBA1C aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BQE3
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TUBA1C also known as tubulin alpha-1C chain is a protein that functions as a component of microtubules which are integral parts of the cytoskeleton in eukaryotic cells. TUBA1C has a molecular mass of approximately 50 kDa. These proteins polymerize to form microtubules essential for various cellular processes such as axonal growth cell division and intracellular transport. TUBA1C is mainly expressed in brain tissues but can also be found in other organ systems indicating its role beyond neuronal function.
- Biological function summary
The tubulin alpha-1C chain plays a significant role in stabilizing cellular structures and facilitating cell division by forming the mitotic spindle. It participates in forming a heterodimer with beta-tubulin a basic structural unit of microtubules. This heterodimer assembles into microtubule filaments which are critical for cell shape and transport mechanisms. TUBA1C as part of the microtubule network helps guide the signaling pathways that are important for neuron functionality and brain development.
- Pathways
TUBA1C is an important player in the mitotic cell cycle and the intracellular transport pathway. It works closely with beta-tubulin to regulate the assembly and disassembly of microtubules which are important steps in cell division and transport within cells. Additionally TUBA1C interacts with MAPs (microtubule-associated proteins) which regulate the dynamics of microtubule systems impacting how cells react to environmental changes and signaling cues during the cell cycle.
- Associated diseases and disorders
Aberrant expression or dysfunction of TUBA1C has been linked to neurodevelopmental disorders and certain types of cancer. Disruption in TUBA1C function can lead to improper neuron migration resulting in conditions such as lissencephaly. Its involvement in cancer relates to its critical role in cell division where abnormal activity can contribute to uncontrolled proliferation. TUBA1C is often examined together with MAPs and other tubulin isoforms in studies related to these diseases to better understand their complex interactions and develop potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-TUBA1C antibody (ab222849)
All lanes: Western blot - Anti-TUBA1C antibody (ab222849) at 1/500 dilution
Lane 1: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 3: U-251 MG (human brain glioma cell line) whole cell lysate
Lane 4: Mouse brain tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1C antibody (ab222849)
Paraffin-embedded human prostate cancer tissue stained for TUBA1C with ab222849 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-TUBA1C antibody (ab222849)
TUBA1C was immunopreciptated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab222849 in K562 whole cell lysate.
Lane 2: ab222849 (4 μg) + K562 whole cell lysate (500 μg)
Lane 3: K562 whole cell lysate 20 μg (Input)
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/50000 dilution.
All lanes: Immunoprecipitation - Anti-TUBA1C antibody (ab222849)
Predicted band size: 50 kDa
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