AB222849

Anti-TUBA1C antibody

Name: Anti-TUBA1C antibody (ab222849) | Abcam
Brand: Abcam Limited
SKU: AB222849
Price: 505 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal TUBA1C antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA1C aa 1-200.

View Alternative Names

TUBA6, TUBA1C, Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1C antibody (AB222849)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1C antibody (AB222849)

Paraffin-embedded human prostate cancer tissue stained for TUBA1C with ab222849 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-TUBA1C antibody (AB222849)

TUBA1C was immunopreciptated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate.

Lane 1 : Rabbit monoclonal IgG (1 μg) instead of ab222849 in K562 whole cell lysate.

Lane 2 : ab222849 (4 μg) + K562 whole cell lysate (500 μg)

Lane 3 : K562 whole cell lysate 20 μg (Input)

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-TUBA1C antibody (ab222849)

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-TUBA1C antibody (AB222849)

All lanes:

Western blot - Anti-TUBA1C antibody (ab222849) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 2:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lane 3:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 4:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human TUBA1C aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BQE3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBA1C also known as tubulin alpha-1C chain is a protein that functions as a component of microtubules which are integral parts of the cytoskeleton in eukaryotic cells. TUBA1C has a molecular mass of approximately 50 kDa. These proteins polymerize to form microtubules essential for various cellular processes such as axonal growth cell division and intracellular transport. TUBA1C is mainly expressed in brain tissues but can also be found in other organ systems indicating its role beyond neuronal function.

Biological function summary

The tubulin alpha-1C chain plays a significant role in stabilizing cellular structures and facilitating cell division by forming the mitotic spindle. It participates in forming a heterodimer with beta-tubulin a basic structural unit of microtubules. This heterodimer assembles into microtubule filaments which are critical for cell shape and transport mechanisms. TUBA1C as part of the microtubule network helps guide the signaling pathways that are important for neuron functionality and brain development.

Pathways

TUBA1C is an important player in the mitotic cell cycle and the intracellular transport pathway. It works closely with beta-tubulin to regulate the assembly and disassembly of microtubules which are important steps in cell division and transport within cells. Additionally TUBA1C interacts with MAPs (microtubule-associated proteins) which regulate the dynamics of microtubule systems impacting how cells react to environmental changes and signaling cues during the cell cycle.

Aberrant expression or dysfunction of TUBA1C has been linked to neurodevelopmental disorders and certain types of cancer. Disruption in TUBA1C function can lead to improper neuron migration resulting in conditions such as lissencephaly. Its involvement in cancer relates to its critical role in cell division where abnormal activity can contribute to uncontrolled proliferation. TUBA1C is often examined together with MAPs and other tubulin isoforms in studies related to these diseases to better understand their complex interactions and develop potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA1C

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:227 PubMed40001145

2025

Revolutionizing the treatment of intervertebral disc degeneration: an approach based on molecular typing.

Applications

Unspecified application

Species

Unspecified reactive species

Shaofeng Chen,Wei Zhang,Yifan Liu,Runzhi Huang,Xiaoyi Zhou,Xianzhao Wei

BMC cancer 23:716 PubMed37528357

2023

TUBA1C is a potential new prognostic biomarker and promotes bladder urothelial carcinoma progression by regulating the cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Jiang,Chao Zhu,Haoxuan Huang,Gaomin Huang,Bin Fu,Xiaoqing Xi

Frontiers in behavioral neuroscience 16:867958 PubMed36172466

2022

Predictors of prodromal Parkinson's disease in young adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Lechner,Jacob M Welsch,Natalie K Pahapill,Taylor A R Kaldenberg,Amy Regenbaum,Cynthia A Kelm-Nelson

Scientific reports 12:7226 PubMed35508511

2022

The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Yamada,Yi Zhong,Shiho Miki,Akiko Taura,Terence H Rabbitts

Cancer cell international 21:144 PubMed33653340

2021

Prognostic biomarker TUBA1C is correlated to immune cell infiltration in the tumor microenvironment of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Bian,Miaosen Zheng,Daishan Jiang,Jian Liu,Hui Sun,Xiaoli Li,Lei Liu,Jianguo Zhang,Yifei Liu

Frontiers in oncology 10:49 PubMed32117719

2020

Upregulated Expression of TUBA1C Predicts Poor Prognosis and Promotes Oncogenesis in Pancreatic Ductal Adenocarcinoma via Regulating the Cell Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Mugahed Abdullah Hasan Albahde,Piao Zhang,Qiuqiang Zhang,Guoqi Li,Weilin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUBA1C antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1C antibody (AB222849)

Immunoprecipitation - Anti-TUBA1C antibody (AB222849)

Western blot - Anti-TUBA1C antibody (AB222849)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise