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Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra), ICC/IF, IP and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (AB177479), expandable thumbnail
  • Western blot - Anti-TUBA4A antibody [EPR13477(B)] (AB177479), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (AB177479), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (AB177479), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (AB177479), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt (Intra)ICC/IFIP
Human
Tested
Tested
Tested
Tested
Expected
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

Recommended products

Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra), ICC/IF, IP and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13477(B)
Purity
Tissue culture supernatant
Specificity

Tubulin proteins have very high homology. The immunogen for this product is based on the TUBA4A protein. There is a chance that this product will detect other tubulin family members. The cross reactivity has not been experimentally determined.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TUBA4A also known as Tubulin Alpha 4A helps in the polymerization of microtubules acting as a core component of the cytoskeleton. It weighs approximately 50 kDa. This protein is expressed in various tissues including the nervous system and muscles. It plays a role in maintaining cellular structure and functions in cellular transport and division.

Biological function summary

Tubulin Alpha 4A is involved in the dynamic assembly and disassembly of microtubules contributing to cell shape and intracellular transport. It forms part of the microtubule complex and interacts with other tubulin isoforms to provide stability and flexibility to microtubule structures. This protein is essential for dividing cells as it supports the mitotic spindle's function during cell division.

Pathways

The TUBA4A protein actively participates in cytoskeleton regulation and signal transduction. It is part of the MAPK signaling pathway important for cell division and differentiation. Components like MAP kinases and related tubulins work together within these pathways to mediate cellular responses to various stimuli facilitating critical cell functions and adaptability.

Associated diseases and disorders

TUBA4A has associations with neurodegenerative conditions and cancers. Mutations in TUBA4A have a link to amyotrophic lateral sclerosis (ALS) where disruptions in microtubule function lead to neuronal damage. Additionally aberrant expression of TUBA4A may contribute to tumor progression where interactions with proteins like beta-tubulin disrupt cellular adhesion and division mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479), expandable thumbnail

    Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab177479, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Predicted band size: 50 kDa

  • Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479), expandable thumbnail

    Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    All lanes: Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479) at 1/1000 dilution

    Lane 1: Molt-4 cell lysate at 10 µg

    Lane 2: A431 cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Lane 5: K562 cell lysate at 10 µg

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunofluorescent analysis of A431 cells labeling TUBA4A with ab177479 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Flow Cytometry (Intracellular) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling TUBA4A with ab177479 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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