Rabbit Polyclonal Alpha-tubulin 4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA4A.
Images
Publications
Filter by
- Biomolecules 12:2022Frontotemporal Lobar Degeneration Case with an N-Terminal Mutation Exhibits Reduced TUBA4A Levels in the Brain and TDP-43 Pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvelien Van Schoor,Mathieu Vandenbulcke,Valérie Bercier,Rik Vandenberghe,Julie van der Zee,Christine Van Broeckhoven,Markus Otto,Bernard Hanseeuw,Philip Van Damme,Ludo Van Den Bosch,Dietmar Rudolf ThalPubMed 35327632
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information. Database link P68366
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Tested | Expected | Expected |
| Sheep | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/20000 | Notes - |
Species Rat | Dilution info 1/500 - 1/20000 | Notes - |
Species Drosophila melanogaster | Dilution info 1/500 - 1/20000 | Notes - |
Species Human | Dilution info 1/500 - 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Xenopus laevis, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Xenopus laevis, Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Xenopus laevis, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain
Recommended products
Rabbit Polyclonal Alpha-tubulin 4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA4A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information. Database link P68366
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TUBA4A also known as Tubulin Alpha 4A helps in the polymerization of microtubules acting as a core component of the cytoskeleton. It weighs approximately 50 kDa. This protein is expressed in various tissues including the nervous system and muscles. It plays a role in maintaining cellular structure and functions in cellular transport and division.
- Biological function summary
Tubulin Alpha 4A is involved in the dynamic assembly and disassembly of microtubules contributing to cell shape and intracellular transport. It forms part of the microtubule complex and interacts with other tubulin isoforms to provide stability and flexibility to microtubule structures. This protein is essential for dividing cells as it supports the mitotic spindle's function during cell division.
- Pathways
The TUBA4A protein actively participates in cytoskeleton regulation and signal transduction. It is part of the MAPK signaling pathway important for cell division and differentiation. Components like MAP kinases and related tubulins work together within these pathways to mediate cellular responses to various stimuli facilitating critical cell functions and adaptability.
- Associated diseases and disorders
TUBA4A has associations with neurodegenerative conditions and cancers. Mutations in TUBA4A have a link to amyotrophic lateral sclerosis (ALS) where disruptions in microtubule function lead to neuronal damage. Additionally aberrant expression of TUBA4A may contribute to tumor progression where interactions with proteins like beta-tubulin disrupt cellular adhesion and division mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody (ab228701)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TUBA4A (green) using ab228701 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody (ab228701)
Paraffin-embedded rat brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract at 20 µg
Lane 2: HEK-293T whole cell lysate/extract at 10 µg
Lane 3: HEK-293T whole cell lysate/extract at 5 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining of Drosophila melanogaster lysate/extract using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/2000 dilution
All lanes: Drosophila melanogaster lysate/extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 2: HeLa cytosolic fraction at 30 µg
Lane 3: HeLa nuclear fraction at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/20000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (rat fibroblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa
Western blot - Anti-TUBA4A antibody - Loading Control (ab228701)
TUBA4A Western blot staining using rabbit Anti-TUBA4A antibody
10% SDS-PAGE
All lanes: Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/20000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: C8D30 whole cell extract at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody (ab228701)
Paraffin-embedded mouse brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com