AB228701

Anti-TUBA4A antibody - Loading Control

Name: Anti-TUBA4A antibody - Loading Control (ab228701) | Abcam
Brand: Abcam Limited
SKU: AB228701
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Alpha-tubulin 4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Drosophila melanogaster, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA4A.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

10 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody - Loading Control (AB228701)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TUBA4A (green) using ab228701 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody - Loading Control (AB228701)

Paraffin-embedded rat brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody - Loading Control (AB228701)

Paraffin-embedded mouse brain tissue stained for TUBA4A with ab228701 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 2:

HeLa cytosolic fraction at 30 µg

Lane 3:

HeLa nuclear fraction at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract at 20 µg

Lane 2:

HEK-293T whole cell lysate/extract at 10 µg

Lane 3:

HEK-293T whole cell lysate/extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/10000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/20000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

C8D30 whole cell extract at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/20000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

true

Supplier Data

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

10% SDS-PAGE

All lanes:

Western blot - Anti-TUBA4A antibody - Loading Control (ab228701) at 1/2000 dilution

All lanes:

Drosophila melanogaster lysate/extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Drosophila melanogaster

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TUBA4A. The exact immunogen used to generate this antibody is proprietary information.

P68366

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBA4A also known as Tubulin Alpha 4A helps in the polymerization of microtubules acting as a core component of the cytoskeleton. It weighs approximately 50 kDa. This protein is expressed in various tissues including the nervous system and muscles. It plays a role in maintaining cellular structure and functions in cellular transport and division.

Biological function summary

Tubulin Alpha 4A is involved in the dynamic assembly and disassembly of microtubules contributing to cell shape and intracellular transport. It forms part of the microtubule complex and interacts with other tubulin isoforms to provide stability and flexibility to microtubule structures. This protein is essential for dividing cells as it supports the mitotic spindle's function during cell division.

Pathways

The TUBA4A protein actively participates in cytoskeleton regulation and signal transduction. It is part of the MAPK signaling pathway important for cell division and differentiation. Components like MAP kinases and related tubulins work together within these pathways to mediate cellular responses to various stimuli facilitating critical cell functions and adaptability.

TUBA4A has associations with neurodegenerative conditions and cancers. Mutations in TUBA4A have a link to amyotrophic lateral sclerosis (ALS) where disruptions in microtubule function lead to neuronal damage. Additionally aberrant expression of TUBA4A may contribute to tumor progression where interactions with proteins like beta-tubulin disrupt cellular adhesion and division mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

One health advances 3:5 PubMed40124736

2025

Arginine as host directed therapy in tuberculosis: insights from modulating arginine metabolism by supplementation and arginase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Qingkui Jiang,Ranjeet Kumar,Yi Zhao,Selvakumar Subbian,Lanbo Shi

Biomolecules 12: PubMed35327632

2022

Frontotemporal Lobar Degeneration Case with an N-Terminal Mutation Exhibits Reduced TUBA4A Levels in the Brain and TDP-43 Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Evelien Van Schoor,Mathieu Vandenbulcke,Valérie Bercier,Rik Vandenberghe,Julie van der Zee,Christine Van Broeckhoven,Markus Otto,Bernard Hanseeuw,Philip Van Damme,Ludo Van Den Bosch,Dietmar Rudolf Thal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUBA4A antibody - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody - Loading Control (AB228701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody - Loading Control (AB228701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Western blot - Anti-TUBA4A antibody - Loading Control (AB228701)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise