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AB154612

Anti-TUBA8 antibody

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(1 Publication)

Rabbit Polyclonal TUBA8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA8 aa 1-300.

View Alternative Names

TUBAL2, TUBA8, Tubulin alpha-8 chain, Alpha-tubulin 8, Tubulin alpha chain-like 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody (AB154612)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody (AB154612)

Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling TUBA8 using ab154612 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TUBA8 antibody (AB154612)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TUBA8 antibody (AB154612)

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TUBA8 using ab154612 at 1/200 dilution. Right panel costained with Hoechst 33342.

Western blot - Anti-TUBA8 antibody (AB154612)
  • WB

Unknown

Western blot - Anti-TUBA8 antibody (AB154612)

10% SDS PAGE

All lanes:

Western blot - Anti-TUBA8 antibody (ab154612) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 50 kDa

false

Western blot - Anti-TUBA8 antibody (AB154612)
  • WB

Unknown

Western blot - Anti-TUBA8 antibody (AB154612)

10% SDS PAGE

All lanes:

Western blot - Anti-TUBA8 antibody (ab154612) at 1/5000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TUBA8 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9NY65

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Primary Antibodies

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Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

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Primary Antibodies

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Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

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Secondary Antibodies

AB150077

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB6160

Anti-Tubulin antibody [YL1/2] - Loading Control

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Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (AB6160)

  • 5
  • 38
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Tris-EDTA buffer (pH 8.0) can also be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBA8 also known as tubulin alpha 8 is a protein involved in maintaining the structure of microtubules. It has a molecular mass of about 50 kDa. This protein serves as an important component of the cytoskeleton helping in cellular shape and intracellular transport. Expression of TUBA8 occurs in various tissues including brain and testes but it is not restricted to these. TUBA8 forms heterodimers with beta-tubulin to construct microtubules playing an essential mechanical role in cell stability and movement.
Biological function summary

TUBA8 contributes to important cellular processes including cell division movement and intracellular transport. It forms part of the microtubule network essential for the dynamic functions of the cytoskeleton. The protein interacts with other tubulin isoforms and various associated proteins facilitating proper cellular function and structure maintenance. By guiding vesicle transport TUBA8 further influences cell signaling and organization.

Pathways

TUBA8 plays an important role in the microtubule assembly pathway and interacts with the mitogen-activated protein kinase (MAPK) pathway. TUBA8's proper functioning is necessary for cellular activities such as mitosis where microtubule dynamics are critical. In the MAPK pathway TUBA8 coordinates with proteins like MAPK1 and MAPK3 impacting cell survival and apoptosis. These interactions show TUBA8's importance in cellular response systems.

Abnormalities in TUBA8 are linked to complex disorders such as lissencephaly and microcephaly. These conditions involve disrupted brain development and abnormal nerve cell positioning highlighting TUBA8's role in neural development. Additionally changes in TUBA8 expression relate to disorders involving the protein LIS1 which also affects microtubule dynamics and brain development. Understanding TUBA8's contribution to these disorders aids in identifying potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
See full target information TUBA8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 139:2154-2163.e5 PubMed31082376

2019

A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Steffen Hörer,Slaheddine Marrakchi,Franz P W Radner,Gerd Zolles,Lisa Heinz,Thomas O Eichmann,Cristina Has,Pavel Salavei,Nadia Mahfoudh,Hamida Turki,Andreas D Zimmer,Judith Fischer
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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