Rabbit Polyclonal TUBA8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA8 aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TUBA8 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY65
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Tris-EDTA buffer (pH 8.0) can also be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBAL2, TUBA8, Tubulin alpha-8 chain, Alpha-tubulin 8, Tubulin alpha chain-like 2
Recommended products
Rabbit Polyclonal TUBA8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBA8 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TUBA8 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY65
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TUBA8 also known as tubulin alpha 8 is a protein involved in maintaining the structure of microtubules. It has a molecular mass of about 50 kDa. This protein serves as an important component of the cytoskeleton helping in cellular shape and intracellular transport. Expression of TUBA8 occurs in various tissues including brain and testes but it is not restricted to these. TUBA8 forms heterodimers with beta-tubulin to construct microtubules playing an essential mechanical role in cell stability and movement.
- Biological function summary
TUBA8 contributes to important cellular processes including cell division movement and intracellular transport. It forms part of the microtubule network essential for the dynamic functions of the cytoskeleton. The protein interacts with other tubulin isoforms and various associated proteins facilitating proper cellular function and structure maintenance. By guiding vesicle transport TUBA8 further influences cell signaling and organization.
- Pathways
TUBA8 plays an important role in the microtubule assembly pathway and interacts with the mitogen-activated protein kinase (MAPK) pathway. TUBA8's proper functioning is necessary for cellular activities such as mitosis where microtubule dynamics are critical. In the MAPK pathway TUBA8 coordinates with proteins like MAPK1 and MAPK3 impacting cell survival and apoptosis. These interactions show TUBA8's importance in cellular response systems.
- Associated diseases and disorders
Abnormalities in TUBA8 are linked to complex disorders such as lissencephaly and microcephaly. These conditions involve disrupted brain development and abnormal nerve cell positioning highlighting TUBA8's role in neural development. Additionally changes in TUBA8 expression relate to disorders involving the protein LIS1 which also affects microtubule dynamics and brain development. Understanding TUBA8's contribution to these disorders aids in identifying potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-TUBA8 antibody (ab154612)
10% SDS PAGE
All lanes: Western blot - Anti-TUBA8 antibody (ab154612) at 1/5000 dilution
All lanes: NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 50 kDa
Western blot - Anti-TUBA8 antibody (ab154612)
10% SDS PAGE
All lanes: Western blot - Anti-TUBA8 antibody (ab154612) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: HeLa whole cell lysate at 30 µg
Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody (ab154612)
Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling TUBA8 using ab154612 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TUBA8 antibody (ab154612)
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling TUBA8 using ab154612 at 1/200 dilution. Right panel costained with Hoechst 33342.
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