Anti-TUBA8 antibody [EPR30576-548]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of paraffin-embedded Embryonic 20 mouse cerebrum tissue labeling TUBA8 with ab325930 at 1/1000 (0.512 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on Embryonic 20 mouse cerebrum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling TUBA8 with ab325930 at 1/1000 (0.512 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on Purkinje cells in mouse cerebellum (PMID : 28388629).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling TUBA8 with ab325930 at 1/1000 (0.512 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse testis.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of paraffin-embedded Embryonic 15.5 mouse cerebrum tissue labeling TUBA8 with ab325930 at 1/1000 (0.512 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on Embryonic 15.5 mouse cerebrum (PMID : 20466094).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh frozen) tissue labeling TUBA8 with ab325930 at 1/50 (10.24 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Panel A : merged staining of anti-TUBA8 (ab325930, green), anti-SPC3 (ab97672, magenta) on mouse testis.
Panel B : anti-TUBA8 stained on mouse testis.
Panel C : anti-SPC3 stained on mouse testis.
The section was incubated with ab325930 and ab97672 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• WB

Lab

Western blot - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, kidney, spleen (PMID : 10772959).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-TUBA8 antibody [EPR30576-548] (ab325930) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 80 µg

Lane 2:

Mouse testis tissue lysate at 80 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 80 µg

Lane 4:

Mouse heart tissue lysate at 80 µg

Lane 5:

Mouse kidney tissue lysate at 80 µg

Lane 6:

Mouse liver tissue lysate at 80 µg

Lane 7:

Mouse spleen tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 180s

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling TUBA8 with ab325930 at 1/50 (10.24 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green).

Negative control : confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab325930 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA8 antibody [EPR30576-548] (AB325930)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TUBA8 with ab325930 at 1/1000 (0.512 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse kidney.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins