AB155311

Anti-TUBB2A + TUBB2B antibody

Name: Anti-TUBB2A + TUBB2B antibody (ab155311) | Abcam
Brand: Abcam Limited
SKU: AB155311

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(1 Publication)

Rabbit Polyclonal TBB2A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUBB2A aa 1-250.

View Alternative Names

TUBB2, TUBB2A, Tubulin beta-2A chain, Tubulin beta class IIa

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (AB155311)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (AB155311)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling beta II Tubulin with ab155311 at 1/200 dilution. Lower panel co-stained with Hoechst 33342 .

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Western blot - Anti-TUBB2A + TUBB2B antibody (AB155311)

10% SDS PAGE

All lanes:

Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

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Western blot - Anti-TUBB2A + TUBB2B antibody (AB155311)

10% SDS PAGE

All lanes:

Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/5000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

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Unknown

Western blot - Anti-TUBB2A + TUBB2B antibody (AB155311)

10% SDS PAGE

All lanes:

Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/3000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TUBB2A aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q13885

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TUBB2A and TUBB2B proteins also known as tubulin beta-2A and beta-2B chains are components of microtubules. These proteins have a molecular mass of approximately 50 kDa each and play roles in the cytoskeleton of cells. TUBB2A and TUBB2B are expressed in various tissues with notable expression in the brain. They participate in the dynamic assembly and disassembly of microtubules which are essential for many cellular processes including maintaining cell shape enabling intracellular transport and facilitating cell division.

Biological function summary

TUBB2A and TUBB2B contribute to the formation of the microtubule network that supports cellular functions. These proteins are integral parts of the cytoskeletal complex interacting with alpha-tubulin to form tubulin dimers. This dimeric structure then polymerizes to build microtubules. Their involvement in mitosis helps ensure proper chromosome segregation which is vital for the accurate transmission of genetic information during cell division. TUBB2A and TUBB2B also support neurite outgrowth and are important for neuronal development and function.

Pathways

The TUBB2A and TUBB2B proteins are essential in the mitotic spindle assembly contributing to the spindle checkpoint pathway. This pathway ensures that chromosomes are correctly aligned and attached to spindle fibers before anaphase begins. They also participate in the regulation of the MAPK/ERK signaling pathway which is important for cell growth and differentiation. Related proteins in these pathways include MAD2 which monitors the spindle assembly checkpoint and RAF a kinase in the MAPK pathway.

Mutations in TUBB2A and TUBB2B have links to cortical dysplasia and polymicrogyria which are brain malformations characterized by disorganized brain tissue. These conditions can lead to developmental delays seizures and cognitive impairments. Additionally the TUBB2A and TUBB2B are related to tau a microtubule-associated protein which when altered relates to neurodegenerative diseases like Alzheimer's. The abnormal function of tau proteins can disrupt microtubule stability and lead to cell death in neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB2A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular endocrinology (Baltimore, Md.) 28:380-94 PubMed24506537

2014

TGFBR1 is required for mouse myometrial development.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Gao,Kayla J Bayless,Qinglei Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUBB2A + TUBB2B antibody

Immunocytochemistry/ Immunofluorescence - Anti-TUBB2A + TUBB2B antibody (AB155311)

Western blot - Anti-TUBB2A + TUBB2B antibody (AB155311)

Western blot - Anti-TUBB2A + TUBB2B antibody (AB155311)

Western blot - Anti-TUBB2A + TUBB2B antibody (AB155311)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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