Rabbit Polyclonal TBB2A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBB2A aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Predicted | Predicted |
Chicken | Predicted | Predicted |
Cow | Predicted | Predicted |
Monkey | Predicted | Predicted |
Pig | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Pig, Monkey, Rat, Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Pig, Monkey, Rat, Chicken, Cow | Dilution info - | Notes - |
Select an associated product type
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Tubulin beta-2B chain
TUBB2, TUBB2, TUBB2A, Tubulin beta-2A chain, Tubulin beta class IIa
Rabbit Polyclonal TBB2A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUBB2A aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This supplementary information is collated from multiple sources and compiled automatically.
The TUBB2A and TUBB2B proteins also known as tubulin beta-2A and beta-2B chains are components of microtubules. These proteins have a molecular mass of approximately 50 kDa each and play roles in the cytoskeleton of cells. TUBB2A and TUBB2B are expressed in various tissues with notable expression in the brain. They participate in the dynamic assembly and disassembly of microtubules which are essential for many cellular processes including maintaining cell shape enabling intracellular transport and facilitating cell division.
TUBB2A and TUBB2B contribute to the formation of the microtubule network that supports cellular functions. These proteins are integral parts of the cytoskeletal complex interacting with alpha-tubulin to form tubulin dimers. This dimeric structure then polymerizes to build microtubules. Their involvement in mitosis helps ensure proper chromosome segregation which is vital for the accurate transmission of genetic information during cell division. TUBB2A and TUBB2B also support neurite outgrowth and are important for neuronal development and function.
The TUBB2A and TUBB2B proteins are essential in the mitotic spindle assembly contributing to the spindle checkpoint pathway. This pathway ensures that chromosomes are correctly aligned and attached to spindle fibers before anaphase begins. They also participate in the regulation of the MAPK/ERK signaling pathway which is important for cell growth and differentiation. Related proteins in these pathways include MAD2 which monitors the spindle assembly checkpoint and RAF a kinase in the MAPK pathway.
Mutations in TUBB2A and TUBB2B have links to cortical dysplasia and polymicrogyria which are brain malformations characterized by disorganized brain tissue. These conditions can lead to developmental delays seizures and cognitive impairments. Additionally the TUBB2A and TUBB2B are related to tau a microtubule-associated protein which when altered relates to neurodegenerative diseases like Alzheimer's. The abnormal function of tau proteins can disrupt microtubule stability and lead to cell death in neurons.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10% SDS PAGE
All lanes: Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/3000 dilution
All lanes: NIH-3T3 whole cell lysate at 30 µg
10% SDS PAGE
All lanes: Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/5000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
10% SDS PAGE
All lanes: Western blot - Anti-TUBB2A + TUBB2B antibody (ab155311) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling beta II Tubulin with ab155311 at 1/200 dilution. Lower panel co-stained with Hoechst 33342 .
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com