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AB170931

Anti-TUBB2A + TUBB2B antibody [EPR11843]

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(6 Publications)

Rabbit Recombinant Monoclonal TBB2A antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

TUBB2, TUBB2A, Tubulin beta-2A chain, Tubulin beta class IIa

4 Images
Flow Cytometry (Intracellular) - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling TUBB2A with ab170931 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling TUBB2A and TUBB2B with ab170931 at 1/100 dilution.

Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)
  • IP

Unknown

Immunoprecipitation - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170931 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Lane 1 Primary : ab170931, 1/10000 dilution
Sample : Immunoprecipitation pellet from 293T cell lysate
Secondary : HRP-conjugated anti-rabbit IgG

Lane 2 Primary : ab170931, 1/10000 dilution
Sample : 1X PBS (negative control)
Secondary : HRP-conjugated anti-rabbit IgG

All lanes:

Immunoprecipitation - Anti-TUBB2A + TUBB2B antibody [EPR11843] (ab170931)

false

Western blot - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)
  • WB

Supplier Data

Western blot - Anti-TUBB2A + TUBB2B antibody [EPR11843] (AB170931)

All lanes:

Western blot - Anti-TUBB2A + TUBB2B antibody [EPR11843] (ab170931) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 50 kDa

false

  • Carrier free

    Anti-TUBB2A+TUBB2B antibody [EPR11843] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11843

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TUBB2A and TUBB2B proteins also known as tubulin beta-2A and beta-2B chains are components of microtubules. These proteins have a molecular mass of approximately 50 kDa each and play roles in the cytoskeleton of cells. TUBB2A and TUBB2B are expressed in various tissues with notable expression in the brain. They participate in the dynamic assembly and disassembly of microtubules which are essential for many cellular processes including maintaining cell shape enabling intracellular transport and facilitating cell division.
Biological function summary

TUBB2A and TUBB2B contribute to the formation of the microtubule network that supports cellular functions. These proteins are integral parts of the cytoskeletal complex interacting with alpha-tubulin to form tubulin dimers. This dimeric structure then polymerizes to build microtubules. Their involvement in mitosis helps ensure proper chromosome segregation which is vital for the accurate transmission of genetic information during cell division. TUBB2A and TUBB2B also support neurite outgrowth and are important for neuronal development and function.

Pathways

The TUBB2A and TUBB2B proteins are essential in the mitotic spindle assembly contributing to the spindle checkpoint pathway. This pathway ensures that chromosomes are correctly aligned and attached to spindle fibers before anaphase begins. They also participate in the regulation of the MAPK/ERK signaling pathway which is important for cell growth and differentiation. Related proteins in these pathways include MAD2 which monitors the spindle assembly checkpoint and RAF a kinase in the MAPK pathway.

Mutations in TUBB2A and TUBB2B have links to cortical dysplasia and polymicrogyria which are brain malformations characterized by disorganized brain tissue. These conditions can lead to developmental delays seizures and cognitive impairments. Additionally the TUBB2A and TUBB2B are related to tau a microtubule-associated protein which when altered relates to neurodegenerative diseases like Alzheimer's. The abnormal function of tau proteins can disrupt microtubule stability and lead to cell death in neurons.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
See full target information TUBB2A
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2023:2826815 PubMed37964983

2023

A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin Liu

Medical science monitor : international medical journal of experimental and clinical research 26:e924343 PubMed33006960

2020

Circular RNA circ_C16orf62 Suppresses Cell Growth in Gastric Cancer by miR-421/Tubulin beta-2A Chain (TUBB2A) Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfeng Jin,Shanshan Zhang,Li Liu

Biochimica et biophysica acta. General subjects 1864:129523 PubMed31935437

2020

Adaptation of striated muscles to Wolframin deficiency in mice: Alterations in cellular bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Kersti Tepp,Marju Puurand,Natalja Timohhina,Jekaterina Aid-Vanakova,Indrek Reile,Igor Shevchuk,Vladimir Chekulayev,Margus Eimre,Nadežda Peet,Lumme Kadaja,Kalju Paju,Tuuli Käämbre

Journal of bioenergetics and biomembranes 50:339-354 PubMed29998379

2018

The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandr Klepinin,Lyudmila Ounpuu,Kati Mado,Laura Truu,Vladimir Chekulayev,Marju Puurand,Igor Shevchuk,Kersti Tepp,Anu Planken,Tuuli Kaambre

Cancer research 76:2824-35 PubMed26980762

2016

Analysis of Liver Tumor-Prone Mouse Models of the Hippo Kinase Scaffold Proteins RASSF1A and SAV1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Zhang,Cai Guo,Xiwei Wu,Arthur X Li,Limin Liu,Walter Tsark,Reinhard Dammann,Hui Shen,Steven L Vonderfecht,Gerd P Pfeifer

Biochemistry and biophysics reports 4:111-125 PubMed29124194

2015

Metabolic remodeling in human colorectal cancer and surrounding tissues: alterations in regulation of mitochondrial respiration and metabolic fluxes.

Applications

Unspecified application

Species

Unspecified reactive species

Vladimir Chekulayev,Kati Mado,Igor Shevchuk,Andre Koit,Andrus Kaldma,Aleksandr Klepinin,Natalja Timohhina,Kersti Tepp,Manana Kandashvili,Lyudmila Ounpuu,Karoliina Heck,Laura Truu,Anu Planken,Vahur Valvere,Tuuli Kaambre
View all publications
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