AB32554

Anti-Tuberin antibody [Y320]

Name: Anti-Tuberin antibody [Y320] (ab32554) | Abcam
Brand: Abcam Limited
SKU: AB32554
Price: 250 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(20 Publications)

Rabbit Recombinant Monoclonal Tuberin antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 20 publications.

View Alternative Names

TSC4, TSC2, Tuberin, Tuberous sclerosis 2 protein

6 Images

Flow Cytometry (Intracellular) - Anti-Tuberin antibody [Y320] (AB32554)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Tuberin antibody [Y320] (AB32554)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Tuberin with purified ab32554 at 1/30 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Tuberin with purified ab32554 at 1/100 dilution (2.97 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Tuberin with purified ab32554 at 1/100 dilution (2.97 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Tuberin with purified ab32554 at 1/100 dilution (2.97 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-Tuberin antibody [Y320] (AB32554)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : Tuberin knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - unpurified ab32554 observed at 180 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab32554 was shown to specifically react with tuberin in wild-type HAP1 cells. No band was observed when tuberin knockout samples were used. Wild-type and tuberin knockout samples were subjected to SDS-PAGE. ab32554 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Tuberin antibody [Y320] (ab32554)

Predicted band size: 201 kDa

false

Unknown

Western blot - Anti-Tuberin antibody [Y320] (AB32554)

The unboiled lysate are recommended to be used in SDS-PAGE, in order to prevent membrane protein aggregation.

All lanes:

Western blot - Anti-Tuberin antibody [Y320] (ab32554) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg

Lane 3:

Mouse brain lysate boiled at 20 µg

Lane 4:

Mouse brain lysate unboiled at 20 µg

Lane 5:

Rat brain lysate boiled at 20 µg

Lane 6:

Rat brain lysate unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 201 kDa

Observed band size: 200 kDa

false

Carrier free

Anti-Tuberin antibody [Y320] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y320

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises Tuberous sclerosis complex-2 (TCS2 also known as Tuberin. It is predicted to detect isoform 2-4 based on sequence homology.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 dilution" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuberin also known as TSC2 is a tumor suppressor protein with a role in cell growth regulation. It has a molecular mass of approximately 200 kDa. This protein forms a critical component of the TSC1-TSC2 complex and is expressed widely in various tissues including the brain kidney heart and skin. Tuberin possesses GTPase-activating protein (GAP) activity targeting specifically the small GTPase protein Rheb which leads to inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

Biological function summary

Tuberin helps control cell size and proliferation. It does this by modulating the mTOR signaling pathway. This modulation is essential for maintaining cellular homeostasis and energy balance particularly under conditions of nutrient scarcity. Tuberin as part of the TSC1-TSC2 complex also influences cell cycle progression and protein synthesis. Its functions are vital in preventing abnormal cell growth and division which is particularly important in organs where rapid cell turnover occurs.

Pathways

Tuberin plays a central role in the mTOR signaling pathway which affects cell growth autophagy and metabolism. It directly interacts with hamartin (TSC1) to form the TSC1-TSC2 complex which regulates Rheb activity. By inhibiting Rheb Tuberin helps control the mTORC1 pathway linking it to signals such as growth factors stress energy status and amino acid availability. This coordination is important for the proper balance between cell growth and catabolism.

Tuberin is mainly associated with Tuberous Sclerosis Complex (TSC) and certain forms of epilepsy. Mutations or loss of function in TSC2 can lead to benign tumor formations in multiple organs including the brain kidneys and skin. It is also implicated in the development of lymphangioleiomyomatosis (LAM) a rare lung disease. Tuberin's interaction with mTORC1 highlights its connection with these conditions as hyperactivation of the mTOR pathway contributes significantly to the pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12906785, PubMed : 15340059, PubMed : 22819219, PubMed : 24529379, PubMed : 28215400, PubMed : 33436626, PubMed : 35772404). Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed : 12172553, PubMed : 12820960, PubMed : 12842888, PubMed : 12906785, PubMed : 15340059, PubMed : 22819219, PubMed : 24529379, PubMed : 33436626). In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12906785, PubMed : 22819219, PubMed : 24529379, PubMed : 28215400, PubMed : 35772404). The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1 (PubMed : 12172553, PubMed : 24529379). Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).

See full target information TSC2

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Journal of cachexia, sarcopenia and muscle 16:e13742 PubMed39962362

2025

Cachexia Alters Central Nervous System Morphology and Functionality in Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Estefania Simoes,Ricardo Uchida,Mariana P Nucci,Fabio L S Duran,Joanna D C C Lima,Leonardo R Gama,Naomi A Costa,Maria C G Otaduy,Fang C Bin,Jose P Otoch,Paulo Alcantara,Alexandre Ramos,Alessandro Laviano,Mauricio Berriel Diaz,Margaret M Esiri,Gabriele C DeLuca,Stephan Herzig,Geraldo Busatto Filho,Marilia Seelaender

IJU case reports 4:127-131 PubMed33977237

2021

Relationship between miR-155 expression and clear cell papillary renal cell carcinoma in the dialyzed kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Ei Shiomi,Renpei Kato,Tomohiko Matsuura,Shigekatsu Maekawa,Yoichiro Kato,Mitsugu Kanehira,Ryo Takata,Jun Sugimura,Kazuyuki Ishida,Takaya Abe,Tamotsu Sugai,Wataru Obara

Neuroscience letters 706:223-230 PubMed31100427

2019

Investigation of the novel mTOR inhibitor AZD2014 in neuronal ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Gina Hadley,Daniel J Beard,Zoi Alexopoulou,Brad A Sutherland,Alastair M Buchan

International journal of biological sciences 15:929-941 PubMed31182914

2019

Feedback Activation of SGK3 and AKT Contributes to Rapamycin Resistance by Reactivating mTORC1/4EBP1 Axis via TSC2 in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao Wang,Fang Huang,Zhe Zhang,Peng Wang,Yuan Luo,Hailiang Li,Na Li,Jian Wang,Jianguang Zhou,Yongan Wang,Shanhu Li

Biomedicine & pharmacotherapy = Biomedecine & phar 112:108599 PubMed30798134

2019

Tanshinone IIA protects against heart failure post-myocardial infarction via AMPKs/mTOR-dependent autophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Zhang,Qiyan Wang,Xiaoping Wang,Xu Chen,Mingyan Shao,Qian Zhang,Dongqing Guo,Yan Wu,Chun Li,Wei Wang,Yong Wang

Neuroscience letters 684:35-41 PubMed29966750

2018

Autophagy mediators (FOXO1, SESN3 and TSC2) in Lewy body disease and aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuo Miki,Kunikazu Tanji,Fumiaki Mori,Jun Utsumi,Hidenao Sasaki,Akiyoshi Kakita,Hitoshi Takahashi,Koichi Wakabayashi

Biochimica et biophysica acta. Molecular cell rese 1865:959-969 PubMed29655803

2018

Role of MSC-derived galectin 3 in the AML microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Peter P Ruvolo,Vivian R Ruvolo,Jared K Burks,YiHua Qiu,Rui-Yu Wang,Elizabeth J Shpall,Leonardo Mirandola,Numsen Hail,Zhihong Zeng,Teresa McQueen,Naval Daver,Sean M Post,Maurizio Chiriva-Internati,Steven M Kornblau,Michael Andreeff

Cancer cell 33:450-462.e10 PubMed29533785

2018

Systematic Functional Annotation of Somatic Mutations in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Kwok-Shing Ng,Jun Li,Kang Jin Jeong,Shan Shao,Hu Chen,Yiu Huen Tsang,Sohini Sengupta,Zixing Wang,Venkata Hemanjani Bhavana,Richard Tran,Stephanie Soewito,Darlan Conterno Minussi,Daniela Moreno,Kathleen Kong,Turgut Dogruluk,Hengyu Lu,Jianjiong Gao,Collin Tokheim,Daniel Cui Zhou,Amber M Johnson,Jia Zeng,Carman Ka Man Ip,Zhenlin Ju,Matthew Wester,Shuangxing Yu,Yongsheng Li,Christopher P Vellano,Nikolaus Schultz,Rachel Karchin,Li Ding,Yiling Lu,Lydia Wai Ting Cheung,Ken Chen,Kenna R Shaw,Funda Meric-Bernstam,Kenneth L Scott,Song Yi,Nidhi Sahni,Han Liang,Gordon B Mills

Autophagy 14:567-583 PubMed29372668

2018

The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Struchtrup,Antonia Wiegering,Björn Stork,Ulrich Rüther,Christoph Gerhardt

Scientific reports 7:4174 PubMed28646232

2017

Inhibition of ERK1/2 Restores GSK3β Activity and Protein Synthesis Levels in a Model of Tuberous Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rituraj Pal,Vitaliy V Bondar,Carolyn J Adamski,George G Rodney,Marco Sardiello

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tuberin antibody [Y320]

Flow Cytometry (Intracellular) - Anti-Tuberin antibody [Y320] (AB32554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [Y320] (AB32554)

Western blot - Anti-Tuberin antibody [Y320] (AB32554)

Western blot - Anti-Tuberin antibody [Y320] (AB32554)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise