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AB133454

Anti-Tuberin (phospho S1254) antibody [EPR5682]

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(2 Publications)

Rabbit Recombinant Monoclonal Tuberin phospho S1254 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

TSC4, TSC2, Tuberin, Tuberous sclerosis 2 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling Tuberin Phospho S1254 with ab133454 at 1/50 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)
  • WB

Unknown

Western blot - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)

All lanes:

Western blot - Anti-Tuberin (phospho S1254) antibody [EPR5682] (ab133454) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate treated with Okadaic Acid and Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 201 kDa

Observed band size: 180 kDa

false

OI-RD Scanning - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5682

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuberin also known as TSC2 is a tumor suppressor protein with a role in cell growth regulation. It has a molecular mass of approximately 200 kDa. This protein forms a critical component of the TSC1-TSC2 complex and is expressed widely in various tissues including the brain kidney heart and skin. Tuberin possesses GTPase-activating protein (GAP) activity targeting specifically the small GTPase protein Rheb which leads to inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
Biological function summary

Tuberin helps control cell size and proliferation. It does this by modulating the mTOR signaling pathway. This modulation is essential for maintaining cellular homeostasis and energy balance particularly under conditions of nutrient scarcity. Tuberin as part of the TSC1-TSC2 complex also influences cell cycle progression and protein synthesis. Its functions are vital in preventing abnormal cell growth and division which is particularly important in organs where rapid cell turnover occurs.

Pathways

Tuberin plays a central role in the mTOR signaling pathway which affects cell growth autophagy and metabolism. It directly interacts with hamartin (TSC1) to form the TSC1-TSC2 complex which regulates Rheb activity. By inhibiting Rheb Tuberin helps control the mTORC1 pathway linking it to signals such as growth factors stress energy status and amino acid availability. This coordination is important for the proper balance between cell growth and catabolism.

Tuberin is mainly associated with Tuberous Sclerosis Complex (TSC) and certain forms of epilepsy. Mutations or loss of function in TSC2 can lead to benign tumor formations in multiple organs including the brain kidneys and skin. It is also implicated in the development of lymphangioleiomyomatosis (LAM) a rare lung disease. Tuberin's interaction with mTORC1 highlights its connection with these conditions as hyperactivation of the mTOR pathway contributes significantly to the pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12906785, PubMed : 15340059, PubMed : 22819219, PubMed : 24529379, PubMed : 28215400, PubMed : 33436626, PubMed : 35772404). Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed : 12172553, PubMed : 12820960, PubMed : 12842888, PubMed : 12906785, PubMed : 15340059, PubMed : 22819219, PubMed : 24529379, PubMed : 33436626). In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12906785, PubMed : 22819219, PubMed : 24529379, PubMed : 28215400, PubMed : 35772404). The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1 (PubMed : 12172553, PubMed : 24529379). Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).
See full target information TSC2 pS1254
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed41008629

2025

LRRK2-Mediated Neuroinflammation-Induced Neuronal Dysfunctions in a Parkinson's and Alzheimer's Disease Cellular Model.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Mutti,Giulia Carini,Moira Marizzoni,Alice Filippini,Federica Bono,Chiara Fiorentini,Samantha Saleri,Floriana De Cillis,Annamaria Cattaneo,Massimo Gennarelli,Paolo Martini,Isabella Russo

Biochimica et biophysica acta. Molecular cell research 1864:113-124 PubMed27984090

2016

RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory Fettweis,Emmanuel Di Valentin,Laurent L'homme,Cédric Lassence,Franck Dequiedt,Marianne Fillet,Isabelle Coupienne,Jacques Piette
View all publications
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Product promise

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