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Rabbit Recombinant Monoclonal Tuberin phospho S664 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

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Images

Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (AB133465), expandable thumbnail
  • OI-RD Scanning - Anti-Tuberin (phospho S664) antibody [EPR8202] (AB133465), expandable thumbnail
  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (AB133465), expandable thumbnail
  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (AB133465), expandable thumbnail
  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (AB133465), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:12172553, PubMed:12271141, PubMed:12842888, PubMed:12906785, PubMed:15340059, PubMed:22819219, PubMed:24529379, PubMed:28215400, PubMed:33436626, PubMed:35772404). Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed:12172553, PubMed:12820960, PubMed:12842888, PubMed:12906785, PubMed:15340059, PubMed:22819219, PubMed:24529379, PubMed:33436626). In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling (PubMed:12172553, PubMed:12271141, PubMed:12842888, PubMed:12906785, PubMed:22819219, PubMed:24529379, PubMed:28215400, PubMed:35772404). The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1 (PubMed:12172553, PubMed:24529379). Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).

Alternative names

TSC4, TSC2, Tuberin, Tuberous sclerosis 2 protein

Recommended products

Rabbit Recombinant Monoclonal Tuberin phospho S664 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8202
Purification technique
Affinity purification Protein A
Dissociation constant
2.12 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tuberin also known as TSC2 is a tumor suppressor protein with a role in cell growth regulation. It has a molecular mass of approximately 200 kDa. This protein forms a critical component of the TSC1-TSC2 complex and is expressed widely in various tissues including the brain kidney heart and skin. Tuberin possesses GTPase-activating protein (GAP) activity targeting specifically the small GTPase protein Rheb which leads to inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

Biological function summary

Tuberin helps control cell size and proliferation. It does this by modulating the mTOR signaling pathway. This modulation is essential for maintaining cellular homeostasis and energy balance particularly under conditions of nutrient scarcity. Tuberin as part of the TSC1-TSC2 complex also influences cell cycle progression and protein synthesis. Its functions are vital in preventing abnormal cell growth and division which is particularly important in organs where rapid cell turnover occurs.

Pathways

Tuberin plays a central role in the mTOR signaling pathway which affects cell growth autophagy and metabolism. It directly interacts with hamartin (TSC1) to form the TSC1-TSC2 complex which regulates Rheb activity. By inhibiting Rheb Tuberin helps control the mTORC1 pathway linking it to signals such as growth factors stress energy status and amino acid availability. This coordination is important for the proper balance between cell growth and catabolism.

Associated diseases and disorders

Tuberin is mainly associated with Tuberous Sclerosis Complex (TSC) and certain forms of epilepsy. Mutations or loss of function in TSC2 can lead to benign tumor formations in multiple organs including the brain kidneys and skin. It is also implicated in the development of lymphangioleiomyomatosis (LAM) a rare lung disease. Tuberin's interaction with mTORC1 highlights its connection with these conditions as hyperactivation of the mTOR pathway contributes significantly to the pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465), expandable thumbnail

    Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465)

    All lanes: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution

    Lane 1: SH SY5Y cell lysates at 10 µg

    Lane 2: SH SY5Y cell lysates treated with Okadaic acid and Calyculin A at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 201 kDa

  • OI-RD Scanning - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465), expandable thumbnail

    OI-RD Scanning - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465), expandable thumbnail

    Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Exposure time:
    Lane 1, 2: 180 seconds
    Lane 3, 4: 40 seconds
    Lane 5, 6: 20 seconds

    Lanes 1 - 6: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution

    Lanes 1 - 6: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] - BSA and Azide free (Anti-Tuberin (phospho S664) antibody [EPR8202] - BSA and Azide free ab248510)

    Lane 1: Human brain lysate unboiled at 15 µg

    Lane 2: Human brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 3: Rat brain lysate unboiled at 15 µg

    Lane 4: Rat brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 5: Mouse brain lysate unboiled at 15 µg

    Lane 6: Mouse brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 200 kDa

  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465), expandable thumbnail

    Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Lanes 1 - 3: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution

    Lanes 1 - 3: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] - BSA and Azide free (Anti-Tuberin (phospho S664) antibody [EPR8202] - BSA and Azide free ab248510)

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled at 15 µg

    Lane 2: HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled at 15 µg

    Lane 3: HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 200 kDa

    Exposure time: 80s

  • Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465), expandable thumbnail

    Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST
    We recommend not to boil the samples after lysis to get desired WB results.
    The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).

    Lanes 1 - 4: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] - BSA and Azide free (Anti-Tuberin (phospho S664) antibody [EPR8202] - BSA and Azide free ab248510)

    Lane 1: Mouse brain lysate boiled at 15 µg

    Lane 2: Mouse brain lysate unboiled at 15 µg

    Lane 3: Rat brain lysate boiled at 15 µg

    Lane 4: Rat brain lysate unboiled at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 200 kDa

    Exposure time: 40s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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