JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB52962

Anti-Tuberin (phospho S939) antibody [EP1062Y]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal Tuberin phospho S939 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

TSC4, TSC2, Tuberin, Tuberous sclerosis 2 protein

2 Images
Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (AB52962)
  • WB

Lab

Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (AB52962)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

This image is derived from purified batch.

All lanes:

Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates at 15 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 201 kDa

Observed band size: 180 kDa

true

Exposure time: 90s

Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (AB52962)
  • WB

Unknown

Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (AB52962)

All lanes:

Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/10000 dilution

Lane 1:

3T3 cell lysate untreated at 10 µg

Lane 2:

3T3 cell lysate treated with insulin at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 201 kDa

Observed band size: 180 kDa

false

  • Carrier free

    Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1062Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuberin also known as TSC2 is a tumor suppressor protein with a role in cell growth regulation. It has a molecular mass of approximately 200 kDa. This protein forms a critical component of the TSC1-TSC2 complex and is expressed widely in various tissues including the brain kidney heart and skin. Tuberin possesses GTPase-activating protein (GAP) activity targeting specifically the small GTPase protein Rheb which leads to inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
Biological function summary

Tuberin helps control cell size and proliferation. It does this by modulating the mTOR signaling pathway. This modulation is essential for maintaining cellular homeostasis and energy balance particularly under conditions of nutrient scarcity. Tuberin as part of the TSC1-TSC2 complex also influences cell cycle progression and protein synthesis. Its functions are vital in preventing abnormal cell growth and division which is particularly important in organs where rapid cell turnover occurs.

Pathways

Tuberin plays a central role in the mTOR signaling pathway which affects cell growth autophagy and metabolism. It directly interacts with hamartin (TSC1) to form the TSC1-TSC2 complex which regulates Rheb activity. By inhibiting Rheb Tuberin helps control the mTORC1 pathway linking it to signals such as growth factors stress energy status and amino acid availability. This coordination is important for the proper balance between cell growth and catabolism.

Tuberin is mainly associated with Tuberous Sclerosis Complex (TSC) and certain forms of epilepsy. Mutations or loss of function in TSC2 can lead to benign tumor formations in multiple organs including the brain kidneys and skin. It is also implicated in the development of lymphangioleiomyomatosis (LAM) a rare lung disease. Tuberin's interaction with mTORC1 highlights its connection with these conditions as hyperactivation of the mTOR pathway contributes significantly to the pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12906785, PubMed : 15340059, PubMed : 22819219, PubMed : 24529379, PubMed : 28215400, PubMed : 33436626, PubMed : 35772404). Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed : 12172553, PubMed : 12820960, PubMed : 12842888, PubMed : 12906785, PubMed : 15340059, PubMed : 22819219, PubMed : 24529379, PubMed : 33436626). In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling (PubMed : 12172553, PubMed : 12271141, PubMed : 12842888, PubMed : 12906785, PubMed : 22819219, PubMed : 24529379, PubMed : 28215400, PubMed : 35772404). The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1 (PubMed : 12172553, PubMed : 24529379). Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).
See full target information TSC2 phospho S939
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 12:1022-1045 PubMed34911733

2021

Ontogeny and Vulnerabilities of Drug-Tolerant Persisters in HER2+ Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chewei Anderson Chang,Jayu Jen,Shaowen Jiang,Azin Sayad,Arvind Singh Mer,Kevin R Brown,Allison M L Nixon,Avantika Dhabaria,Kwan Ho Tang,David Venet,Christos Sotiriou,Jiehui Deng,Kwok-Kin Wong,Sylvia Adams,Peter Meyn,Adriana Heguy,Jane A Skok,Aristotelis Tsirigos,Beatrix Ueberheide,Jason Moffat,Abhyudai Singh,Benjamin Haibe-Kains,Alireza Khodadadi-Jamayran,Benjamin G Neel

Scientific reports 11:7787 PubMed33833339

2021

Oncogenic role of MiR-130a in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik Mallela,Swamy Shivananda,Kodaganur S Gopinath,Arun Kumar

Digestive diseases and sciences 65:1689-1699 PubMed31863340

2019

Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Tang,Yi Zeng,Lei Li,Jingjing Wang,Duo Peng,Ting Zhang,Hongxing Zhang,Xue An

Oncogene 27:6690-7 PubMed18695678

2008

The G1556S-type tuberin variant suppresses tumor formation in tuberous sclerosis 2 mutant (Eker) rats despite its deficiency in mTOR inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

M Shiono,T Kobayashi,R Takahashi,G Sun,M Abe,D Zhang,L Wang,X Piao,Y Takagi,R Mineki,H Taka,N Tada,S Sonobe,S Momose,M Ueda,O Hino
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB133454

Anti-Tuberin (phospho S1254) antibody [EPR5682]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho S1254) antibody [EPR5682] (AB133454)

  • 0
  • 0
Primary Antibodies

AB32554

Anti-Tuberin antibody [Y320]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Tuberin antibody [Y320] (AB32554)

  • 5
  • 1
Primary Antibodies

AB109403

Anti-Tuberin (phospho T1462) antibody [EPR1060(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (AB109403)

  • 0
  • 0
Primary Antibodies

AB133465

Anti-Tuberin (phospho S664) antibody [EPR8202]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Tuberin (phospho S664) antibody [EPR8202] (AB133465)

  • 0
  • 0
Primary Antibodies

AB178441

Anti-Tuberin antibody [Y320] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Tuberin antibody [Y320] - BSA and Azide free (AB178441)

  • 0
  • 0
Primary Antibodies

AB52936

Anti-Tuberin antibody [EP1107Y]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin antibody [EP1107Y] (AB52936)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133323

Anti-14-3-3 antibody [EPR6379]

RabMAb|Recombinant

Western blot - Anti-14-3-3 antibody [EPR6379] (AB133323)

  • 0
  • 0
Primary Antibodies

AB109268

Anti-mTOR (phospho S2448) antibody [EPR426(2)]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

  • 4
  • 6
Primary Antibodies

AB206292

Anti-14-3-3 eta/YWHAH antibody [EPR16750]

RabMAb|Recombinant

Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] (AB206292)

  • 5
  • 2
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com