AB129021

Anti-TUBG1 antibody [EPR8449] - Centrosome Marker

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TBG1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1

2 Images

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Western blot - Anti-TUBG1 antibody [EPR8449] - Centrosome Marker (AB129021)

All lanes:

Western blot - Anti-TUBG1 antibody [EPR8449] - Centrosome Marker (ab129021) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

A431 cell lysates at 10 µg

Lane 4:

K562 cell lysates at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa

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OI-RD Scanning - Anti-TUBG1 antibody [EPR8449] - Centrosome Marker (AB129021)

OI-RD Scanning

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OI-RD Scanning - Anti-TUBG1 antibody [EPR8449] - Centrosome Marker (AB129021)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-TUBG1 antibody [EPR8449] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8449

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBG1 also known as gamma-tubulin plays an important role in the nucleation of microtubules. It acts as a vital component in the formation of microtubule-organizing centers such as centrosomes and spindle pole bodies. Gamma-tubulin typically has a molecular mass of around 48 kDa. Expression of TUBG1 occurs in various tissues with notable presence in brain and testes reflecting its importance in cells undergoing rapid division and developmental processes.

Biological function summary

Gamma-tubulin forms part of the gamma-tubulin ring complex which facilitates the initiation of microtubule assembly. This protein complex binds to microtubule minus ends and provides a template assisting in polymerization. Through this action TUBG1 indirectly influences cell shape motility and division. The gamma-tubulin ring complex therefore ensures proper mitotic spindle formation essential for accurate chromosomal segregation during cell division.

Pathways

Multiple cellular processes such as mitotic spindle assembly and cell cycle progression rely on the function of TUBG1. This protein integrates into the spindle assembly checkpoint pathway ensuring chromosomes attach correctly to spindle fibers before segregation. It's also implicated in the microtubule nucleation pathway working alongside other proteins like TUBGCP2 and NEDD1 to maintain microtubule dynamics and cellular organization.

Mutations or dysregulation of TUBG1 links to cancer and certain neurological conditions. Alterations in TUBG1 expression can contribute to tumor development given its role in cell cycle control and division. Moreover TUBG1 mutations associate with brain malformations such as lissencephaly where gamma-tubulin interacts with proteins like LIS1 involved in neuronal migration. Understanding TUBG1's contributions to both normal cellular processes and disease states can offer insights into new therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 38305685, PubMed : 38609661, PubMed : 39321809). Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation (PubMed : 38305685, PubMed : 38609661, PubMed : 39321809). The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation (PubMed : 38305685, PubMed : 38609661, PubMed : 39321809).

See full target information TUBG1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 110:2792-2798 PubMed40468949

2025

Characterization of a novel mutation in a family with thrombocytopenia and other congenital malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Ammeti,Serena Barozzi,Alessandro Pecci,Melania Eva Zanchetta,Edward Cesnik,Alessandra Ferlini,Mariabeatrice Sanchini,Laura Verga,Valeria Bozzi,Fabio Corsolini,Michela Faleschini,Anna Savoia,Stefania Bigoni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUBG1 antibody [EPR8449] - Centrosome Marker

Western blot - Anti-TUBG1 antibody [EPR8449] - Centrosome Marker (AB129021)

OI-RD Scanning - Anti-TUBG1 antibody [EPR8449] - Centrosome Marker (AB129021)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise