Anti-TUBGCP2 antibody [GCP2-01]

• Flow Cyt

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Flow Cytometry - Anti-TUBGCP2 antibody [GCP2-01] (AB140225)

Overlay histogram showing HeLa cells stained with ab140225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140225, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• WB

Supplier Data

Western blot - Anti-TUBGCP2 antibody [GCP2-01] (AB140225)

Western blotting analysis of TUBGCP2 using ab140225 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 μg/ml of ab140225 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.

All lanes:

Western blot - Anti-TUBGCP2 antibody [GCP2-01] (ab140225)

Predicted band size: 103 kDa

Observed band size: 95 kDa

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