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Mouse Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 42 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TUBA1B acetyl K40.


Images

Western blot - Anti-Tubulin antibody [4D1] - Loading Control (AB56676), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [4D1] - Loading Control (AB56676), expandable thumbnail
  • Flow Cytometry - Anti-Tubulin antibody [4D1] - Loading Control (AB56676), expandable thumbnail
  • Western blot - Anti-Tubulin antibody [4D1] - Loading Control (AB56676), expandable thumbnail

Publications

  • JCI insight 7:2022
    A single-domain i-body, AD-114, attenuates renal fibrosis through blockade of CXCR4.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Qinghua Cao,Chunling Huang,Hao Yi,Anthony J Gill,Angela Chou,Michael Foley,Chris G Hosking,Kevin K Lim,Cristina F Triffon,Ying Shi,Xin-Ming Chen,Carol A Pollock
    PubMed 35015734
  • Molecular therapy oncolytics 23:582-5922021
    Improved lentiviral vector titers from a multi-gene knockout packaging line.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jiaying Han,Kevin Tam,Curtis Tam,Roger P Hollis,Donald B Kohn
    PubMed 34938858

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TUBA1B acetyl K40. Database link P68363

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

    Target data

    Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    Alternative names

    Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

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    Mouse Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 42 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TUBA1B acetyl K40.

    Alternative names

    Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

    Key facts

    Isotype

    IgG2b

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • Recombinant Full Length Protein corresponding to Human TUBA1B acetyl K40. Database link P68363
    Clone number

    4D1

    Purity

    Tissue culture supernatant

    Light chain type

    kappa

    Concentration
    Loading...
    Purification notes

    Purified from TCS.

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

    If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

    Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

    Supplementary info

    Activity summary

    Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.

    Biological function summary

    This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.

    Pathways

    Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.

    Associated diseases and disorders

    Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    4 product images

    • Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676), expandable thumbnail

      Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)

      Tubulin antibody (ab56676) at 1ug/lane + A-431 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

      All lanes: Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [4D1] - Loading Control (ab56676), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)

      Tubulin antibody (ab56676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human prostate.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-Tubulin antibody [4D1] - Loading Control (ab56676), expandable thumbnail

      Flow Cytometry - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)

      Overlay histogram showing HeLa cells stained with ab56676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56676, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
      This image was generated using the ascites version of the product.

    • Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676), expandable thumbnail
      This image is courtesy of a customer review submitted by Xun Ai

      Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)

      Blocking Step : 5% BSA for 2 hours at 23°C

      This image was generated using the ascites version of the product.

      All lanes: Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676) at 1 µg/mL

      Lane 1: Whole tissue lysate of rabbit heart at 20 µg

      Lane 2: Whole tissue lysate of rabbit heart at 40 µg

      Lane 3: Whole tissue lysate of rabbit heart at 60 µg

      Lane 4: Whole tissue lysate of rabbit heart at 80 µg

      Lane 5: Whole tissue lysate of rabbit heart at 100 µg

      Lane 6: Whole tissue lysate of rabbit heart at 120 µg

      Secondary

      All lanes: An HRP-conjugated Donkey anti-mouse IgG polyclonal at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 50 kDa

      Observed band size: 47 kDa

      Exposure time: 1s

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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