AB210797

Anti-Tubulin antibody [EPR13796] - Loading Control

RabMAb
Recombinant
Lab Essentials
What is this?

(1 Review)

(40 Publications)

Rabbit Recombinant Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae, Saccharomyces cerevisiae samples. Cited in 40 publications.

View Alternative Names

Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

2 Images

Supplier Data

Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (AB210797)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution

All lanes:

Saccharomyces cerevisiae whole cell lysates at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (AB210797)

Blocking/Dilution buffer : 5% NFDM/TBST.

Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain contains aa1-445 with a His-Tag® was made in house.

All lanes:

Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (ab210797) at 1/1000 dilution

All lanes:

Full length recombinant protein Saccharomyces cerevisiae tubulin alpha-3 chain at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 3min

Carrier free

Anti-Tubulin antibody [EPR13796] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13796

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab210797 recognizes Tubulin alpha-1 and alpha-3 chains.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.

Biological function summary

This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.

Pathways

Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.

Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed : 34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871).

See full target information TUBA1B acetyl K40

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70030 PubMed40909350

2025

Epigenetic regulation of breast ductal carcinoma in situ by miR-217 through DNMT1 and Hedgehog-GLI pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zixin Wang,Liangping Wu,Shuhui Lai,Sixuan Guo,Changqin Pu,Linyi Zhang,Xiaoling Li

Cancer science 115:2673-2685 PubMed38801832

2024

Hexokinase 2 nonmetabolic function-mediated phosphorylation of IκBα enhances pancreatic ductal adenocarcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Tong,Xin Liu,Lihui Wu,Yaoxian Xiang,Jing Wang,Yurong Cheng,Chan Zhang,Baojuan Han,Li Wang,Dong Yan

Human cell 36:1755-1772 PubMed37336868

2023

PSMD4 drives progression of hepatocellular carcinoma via Akt/COX2 pathway and p53 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Zhang,Shu Fang,Fanghao Rong,Miaomiao Jia,Yunpeng Wang,Huixian Cui,Peipei Hao

Cell research 33:712-726 PubMed37188880

2023

Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Xupeng Liu,Ziyue Yao,Yu Chen,Lanfang Luo,Kun Liang,Jun-Hao Elwin Tan,Min-Wen Jason Chua,Yan-Jiang Benjamin Chua,Shilin Ma,Liping Zhang,Wenwu Ma,Shuqing Liu,Wenhua Cao,Luyao Guo,Lu Guang,Yuefan Wang,He Zhao,Na Ai,Yun Li,Chunwei Li,Ruiqi Rachel Wang,Bin Tean Teh,Lan Jiang,Kang Yu,Ng Shyh-Chang

Cell proliferation 56:e13459 PubMed37177849

2023

Adult progenitor rejuvenation with embryonic factors.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Xupeng Liu,Yu Chen,Elwin Tan Jun-Hao,Ziyue Yao,Jason Chua Min-Wen,Benjamin Chua Yan-Jiang,Shilin Ma,Wenwu Ma,Lanfang Luo,Luyao Guo,Dan Song,Ng Shyh-Chang

Journal for immunotherapy of cancer 11: PubMed36796878

2023

PKR induces TGF-β and limits oncolytic immune therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Bangxing Hong,Upasana Sahu,Matthew P Mullarkey,Evan Hong,Guangsheng Pei,Yuanqing Yan,Yoshihiro Otani,Yeshavanth Banasavadi-Siddegowda,Huihui Fan,Zhongming Zhao,Jianhua Yu,Michael A Caligiuri,Balveen Kaur

Neuroscience letters 792:136941 PubMed36367512

2022

Annexin A2 promotes angiogenesis after ischemic stroke via annexin A2 receptor - AKT/ERK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Lin,Wenlu Li,Zexu Shen,Yun Bei,Taofeng Wei,Zhanyang Yu,Yunjian Dai,Haibin Dai

Cell death & disease 13:413 PubMed35484132

2022

Hypoxia-induced NFATc3 deSUMOylation enhances pancreatic carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Tong,Zheng Zhang,Yurong Cheng,Jing Yang,Cong Fan,Xuyang Zhang,Jiandong Yang,Li Wang,Dong Guo,Dong Yan

Experimental and therapeutic medicine 23:48 PubMed34917179

2021

HIF-1α mediates the protective effect of plasma extracellular particles induced by remote ischaemic preconditioning on oxidative stress injury in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minghua Wen,Feng Hu,Zhijian Gong,Chahua Huang,Xiaoshu Cheng

Frontiers in oncology 11:754353 PubMed34722307

2021

MiR-629-5p Promotes Prostate Cancer Development and Metastasis by Targeting AKAP13.

Applications

Unspecified application

Species

Unspecified reactive species

Yangzhou Liu,Shankun Zhao,Jiamin Wang,Zhiguo Zhu,Lianmin Luo,Qian Xiang,Mingda Zhou,Yuxiang Ma,Zuomin Wang,Zhigang Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tubulin antibody [EPR13796] - Loading Control

Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (AB210797)

Western blot - Anti-Tubulin antibody [EPR13796] - Loading Control (AB210797)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (40)

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