AB185224

Anti-Tubulin antibody [EPR13797] - Loading Control

RabMAb
Recombinant
Lab Essentials
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Alpha-tubulin 1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

View Alternative Names

Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

1 Images

Supplier Data

Western blot - Anti-Tubulin antibody [EPR13797] - Loading Control (AB185224)

All lanes:

Western blot - Anti-Tubulin antibody [EPR13797] - Loading Control (ab185224) at 1/5000 dilution

All lanes:

Saccharomyces cerevisiae lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

false

Carrier free

Anti-Tubulin antibody [EPR13797] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13797

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.

Biological function summary

This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.

Pathways

Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.

Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 38305685, PubMed : 34996871, PubMed : 38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 38305685, PubMed : 34996871, PubMed : 38609661). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871, PubMed : 38609661).

See full target information TUBA1B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5853 PubMed37730811

2023

Genome-wide probing of eukaryotic nascent RNA structure elucidates cotranscriptional folding and its antimutagenic effect.

Applications

Unspecified application

Species

Unspecified reactive species

Gongwang Yu,Yao Liu,Zizhang Li,Shuyun Deng,Zhuoxing Wu,Xiaoyu Zhang,Wenbo Chen,Junnan Yang,Xiaoshu Chen,Jian-Rong Yang

PLoS genetics 17:e1009539 PubMed33914734

2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Gersing,Yong Wang,Martin Grønbæk-Thygesen,Caroline Kampmeyer,Lene Clausen,Martin Willemoës,Claes Andréasson,Amelie Stein,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

Pigment cell & melanoma research 33:16-29 PubMed31386789

2019

A ROCK inhibitor promotes keratinocyte survival and paracrine secretion, enhancing establishment of primary human melanocytes and melanocyte-keratinocyte co-cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Mi,Yang Feng,Jie Wen,Yiqun Su,Lin Xu,Tingjian Zu,Chang Liu,David E Fisher,Xunwei Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tubulin antibody [EPR13797] - Loading Control

Western blot - Anti-Tubulin antibody [EPR13797] - Loading Control (AB185224)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise