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Anti-Tubulin antibody [YL1/2] ab6160 is a rat monoclonal antibody that is used in Tubulin western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Antibody clone YL1/2 has been tried and trusted by researchers since 2002 and is cited in >610 publications
- One antibody for all your Tubulin staining, use in Tubulin western blotting, IHC, immunofluorescence and flow cytometry


Images

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (AB6160), expandable thumbnail
  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (AB6160), expandable thumbnail
  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (AB6160), expandable thumbnail
  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (AB6160), expandable thumbnail
  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (AB6160), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Rat

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected
African green monkey
Predicted
Predicted
Predicted
Predicted
Caenorhabditis elegans
Predicted
Predicted
Predicted
Predicted
Drosophila melanogaster
Predicted
Predicted
Predicted
Predicted
Mammals
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted
Saccharomyces cerevisiae
Predicted
Predicted
Predicted
Predicted
Schizosaccharomyces pombe
Predicted
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Mammals, African green monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000.00000 - 1/10000.00000

Notes

-

Species

Rat

Dilution info

1/5000.00000 - 1/10000.00000

Notes

-

Species

Human

Dilution info

1/5000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Mammals, African green monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Mammals, African green monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

(see PMID: 16230461)

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Mammals, African green monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Alternative names

Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

Recommended products

Anti-Tubulin antibody [YL1/2] ab6160 is a rat monoclonal antibody that is used in Tubulin western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Antibody clone YL1/2 has been tried and trusted by researchers since 2002 and is cited in >610 publications
- One antibody for all your Tubulin staining, use in Tubulin western blotting, IHC, immunofluorescence and flow cytometry

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

YL1/2

Purification technique

Affinity purification Protein G

Epitope

The YL1/2 monoclonal epitope has been mapped to the last 8 residues (GEEEGEEY) at the carboxy terminus of alpha tubulin when tyrosinated (PubMed IDs: 6415068, 6204858).

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.

Biological function summary

This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.

Pathways

Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.

Associated diseases and disorders

Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (Alexa Fluor® 790) ab175786 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa Whole Cell Lysate at 10 µg/mL

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (Alexa Fluor® 790) (Goat Anti-Rat IgG H&L (Alexa Fluor® 790) ab175786) at 1/10000 dilution

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed ab175750 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed (Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed ab175750) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (Alexa Fluor® 750) ab175751 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (Alexa Fluor® 750) (Goat Anti-Rat IgG H&L (Alexa Fluor® 750) ab175751) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed ab175777 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 5 µg

    Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 2 µg

    Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 1 µg

    Secondary

    All lanes: Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed (Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed ab175777) at 1/10000 dilution

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (Alexa Fluor® 680) ab175778 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (Alexa Fluor® 680) (Goat Anti-Rat IgG H&L (Alexa Fluor® 680) ab175778) at 1/10000 dilution

    Predicted band size: 36 kDa

    Observed band size: 50 kDa

  • Flow Cytometry (Intracellular) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab6160 (red line).

    The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6160, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450, 1 μg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    ICC/IF image of ab6160 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed in 100% methanol 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165 Alexa Fluor® 488 goat anti-rat IgG (H+L) pre-adsorbed, used at a 1/1000 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 minutes).

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    Lanes 1 and 3: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1/5000 dilution

    Lanes 2 and 4: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1/10000 dilution

    Lanes 1 - 2: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg

    Lanes 3 - 4: Western blot - BALB/3T3 whole cell lysate (BALB/3T3 whole cell lysate ab7901) at 20 µg

    Secondary

    All lanes: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (Rabbit Anti-Rat IgG H&L (HRP) ab6734) at 1/2000 dilution

    Predicted band size: 50 kDa

    Observed band size: 17 kDa, 34 kDa, 52 kDa, 80 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail
    Loison-Robert et al PLoS One. 2018 Jan 25;13(1):e0190014. doi: 10.1371/journal.pone.0190014. eCollection 2018. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    Cytoskeleton and major extracellular matrix proteins in human DPSC (Dental pulp stem cell) were analyzed by immunofluorescence.

    Vimentin and tubulin (Panel D, control, E, (BD) and F, (BR)) are shown.

    After 7 days in contact with/without the materials (Biodentine (BD) and Bioroot (BR)), coated coverslip cultures were fixed in PBS (pH 7.4) containing 4% paraformaldehyde/5% sucrose for 10 minutes. For detection of intracellular molecules, the cells on the coverslips were permeabilized using 0.5% Triton X-100. To block background staining, cells were treated with PBS containing 1% BSA/1% glycine at 37°C for 20 minutes. Samples were incubated with the primary antibody at 4°C overnight or at 37°C for 2 hours. For double immunostaining, primary antibodies were incubated as above. Samples were then incubated with the appropriate secondary antibodies at 37°C for 1 hour. Cell nuclei were stained using DAPI.

    Scale Bar: 100 μm.

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) whole cell lysate at 10 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 3: PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase Conjugated Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa

    Observed band size: 52 kDa, 85 kDa

    Exposure time: 8min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    IHC image of Tubulin staining in human colon formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6 for 20 minutes. The section was then incubated with ab6160, 5 μg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (Goat Anti-Rat IgG H&L (Alkaline Phosphatase) ab6846) at 1/5000 dilution

    Lanes 3 - 4: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (Goat Anti-Rat IgG H&L (Alkaline Phosphatase) ab6846) at 1/20000 dilution

    Lanes 5 - 6: Western blot - Goat Anti-Rat IgG H&L (Alkaline Phosphatase) (Goat Anti-Rat IgG H&L (Alkaline Phosphatase) ab6846) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab97057) at 1/2000 dilution

    Lanes 3 - 4: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab97057) at 1/10000 dilution

    Lanes 5 - 6: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab97057) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) ab102169) at 1/2000 dilution

    Lanes 3 - 4: Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) ab102169) at 1/10000 dilution

    Lanes 5 - 6: Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) ab102169) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    Lanes 1 - 6: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    Lane 5: Anti-RUNX2 antibody (ab102712) at 1/20000 dilution

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (Rabbit Anti-Rat IgG H&L (HRP) ab102172) at 1/2000 dilution

    Lanes 3 - 4: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (Rabbit Anti-Rat IgG H&L (HRP) ab102172) at 1/10000 dilution

    Lane 6: Western blot - Rabbit Anti-Rat IgG H&L (HRP) (Rabbit Anti-Rat IgG H&L (HRP) ab102172) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160), expandable thumbnail

    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/mL

    All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) ab6735) at 1/2000 dilution

    Lanes 3 - 4: Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) ab6735) at 1/10000 dilution

    Lanes 5 - 6: Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) ab6735) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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