Name: Anti-Tubulin antibody [YOL1/34] - Loading Control
SKU: ab6161
Rating: 5 (21 reviews)

Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Microtubule marker. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Rat, Mouse, Human samples. Cited in 156 publications.

Images

Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB6161), expandable thumbnail

Publications

Key facts

Isotype: IgG2a
Host species: Rat
Storage buffer: pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	Flow Cyt (Intra)	WB
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Tested
Rat	Tested	Expected	Tested
Alligator	Predicted	Predicted	Predicted
Dog	Predicted	Predicted	Predicted
Mammals	Predicted	Predicted	Predicted
Plants	Predicted	Predicted	Predicted
Saccharomyces cerevisiae	Predicted	Predicted	Predicted
Schizosaccharomyces pombe	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 5 µg/mL	Notes -
Species Mouse	Dilution info 5 µg/mL	Notes -
Species Human	Dilution info 5 µg/mL	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, Mammals, Alligator	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg for 10⁶ Cells	Notes Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, Mammals, Alligator	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1 µg/mL	Notes -
Species Rat	Dilution info 1 µg/mL	Notes -
Species Human	Dilution info 1 µg/mL	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, Mammals, Alligator	Dilution info -	Notes -

Associated Products

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9 products for Alternative Product

Target data

See full target information TUBA1B acetyl K40

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).

Alternative names

Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

Recommended products

Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Microtubule marker. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Rat, Mouse, Human samples. Cited in 156 publications.

Key facts

Isotype: IgG2a
Host species: Rat
Storage buffer: pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: YOL1/34
Purification technique: Affinity purification Protein G
Epitope: ab6161 binds to an epitope between amino acids 414 and 422 of alpha tubulin.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

We can conjugate this antibody to FITC for you (please see ab150252 for details). This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.

Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.

Biological function summary

This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.

Pathways

Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.

Associated diseases and disorders

Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.

Product promise

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10 product images

This image is courtesy of an anonymous customer review
Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. An Alexa Fluor^® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary.
Flow Cytometry (Intracellular) - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
Overlay histogram showing HeLa cells stained with ab6161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6161, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Tubulin Western blot staining using rat Anti-Tubulin antibody
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (HRP) ab205720, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 54 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
ab6161 staining Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6161 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Tubulin Western blot staining using rat Anti-Tubulin antibody
Western blot against tubulin with ab6161 at 1/3000. Secondary Rabbit anti-Rat IgG HRP (Rabbit Anti-Rat IgG H&L (HRP) ab6734)was used at 1/2000. Exposure time: 2mins.
Lane 1: 20μg/lane HeLa (Human) whole cell lysates (ab7898).
Lane 2: 20μg/lane 3T3 (Mouse) whole cell lysate (BALB/3T3 whole cell lysate ab7901).
Lane 3: 20μg/lane Rat brain tissue lysate (ab7942).
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Predicted band size: 50 kDa
This image is courtesy of an anonymous customer review
Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Tubulin Western blot staining of Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating using rat Anti-Tubulin antibody
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1/2000 dilution
All lanes: Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating at 5 µg
Secondary
All lanes: HRP conjugated goat anti-rat antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30s
Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Tubulin Western blot staining of Brain (Rat) Tissue Lysate using rat Anti-Tubulin antibody
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1 µg/mL
All lanes: Brain (Rat) Tissue Lysate at 10 µg
Secondary
All lanes: Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa, 55 kDa
Observed band size: 54 kDa
Exposure time: 3min
Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Tubulin Western blot staining using rat Anti-Tubulin antibody
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (HRP) ab205720 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
Secondary
All lanes: Goat Anti-Rat IgG H&L (HRP) ab205720 (Left Image) at 1/5000 and a competitor secondary (Right Image) at 1/5000. Notice the increased background of the competitor product.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 54 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
ab6161 staining tubulin HeLa cells treated with anisomycin (Anisomycin, Protein synthesis inhibitor ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of Anisomycin, Protein synthesis inhibitor ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.