Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Microtubule marker. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Rat, Mouse, Human samples. Cited in 156 publications.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
ICC/IF | Flow Cyt (Intra) | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Tested |
Rat | Tested | Expected | Tested |
Alligator | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted |
Mammals | Predicted | Predicted | Predicted |
Plants | Predicted | Predicted | Predicted |
Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
Schizosaccharomyces pombe | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, Mammals, Alligator | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, Mammals, Alligator | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, Mammals, Alligator | Dilution info - | Notes - |
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Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).
Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B
Rat Monoclonal Alpha-tubulin 1 acetyl K40 antibody. Microtubule marker. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Rat, Mouse, Human samples. Cited in 156 publications.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
We can conjugate this antibody to FITC for you (please see ab150252 for details). This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.
Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.
This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.
Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.
Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. An Alexa Fluor® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary.
Overlay histogram showing HeLa cells stained with ab6161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6161, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Tubulin Western blot staining using rat Anti-Tubulin antibody
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (HRP) ab205720, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 54 kDa
Exposure time: 15s
ab6161 staining Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6161 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Tubulin Western blot staining using rat Anti-Tubulin antibody
Western blot against tubulin with ab6161 at 1/3000. Secondary Rabbit anti-Rat IgG HRP (Rabbit Anti-Rat IgG H&L (HRP) ab6734)was used at 1/2000. Exposure time: 2mins.
Lane 1: 20μg/lane HeLa (Human) whole cell lysates (ab7898).
Lane 2: 20μg/lane 3T3 (Mouse) whole cell lysate (BALB/3T3 whole cell lysate ab7901).
Lane 3: 20μg/lane Rat brain tissue lysate (ab7942).
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161)
Predicted band size: 50 kDa
Tubulin Western blot staining of Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating using rat Anti-Tubulin antibody
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1/2000 dilution
All lanes: Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating at 5 µg
All lanes: HRP conjugated goat anti-rat antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30s
Tubulin Western blot staining of Brain (Rat) Tissue Lysate using rat Anti-Tubulin antibody
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1 µg/mL
All lanes: Brain (Rat) Tissue Lysate at 10 µg
All lanes: Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa, 55 kDa
Observed band size: 54 kDa
Exposure time: 3min
Tubulin Western blot staining using rat Anti-Tubulin antibody
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6161 overnight at 4°C. Antibody binding was detected using Goat Anti-Rat IgG H&L (HRP) ab205720 (Left Image) and a competitor secondary (Right Image), and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Tubulin antibody [YOL1/34] - Loading Control (ab6161) at 1 µg/mL
Lane 1: Liver (Human) Tissue Lysate at 10 µg
Lane 2: Liver (Mouse) Tissue Lysate at 10 µg
Lane 3: Liver (Rat) Tissue Lysate at 10 µg
All lanes: Goat Anti-Rat IgG H&L (HRP) ab205720 (Left Image) at 1/5000 and a competitor secondary (Right Image) at 1/5000. Notice the increased background of the competitor product.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 54 kDa
Exposure time: 15s
ab6161 staining tubulin HeLa cells treated with anisomycin (Anisomycin, Protein synthesis inhibitor ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of Anisomycin, Protein synthesis inhibitor ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.
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