AB67991

Anti-TUFM antibody

Name: Anti-TUFM antibody (ab67991) | Abcam
Brand: Abcam Limited
SKU: AB67991
Price: 535 USD
Availability: InStock

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(5 Publications)

Mouse Polyclonal TUFM antibody. Carrier free. Suitable for WB and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TUFM.

View Alternative Names

EF-Tu, P43, TUFM

1 Images

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Western blot - Anti-TUFM antibody (AB67991)

All lanes:

Western blot - Anti-TUFM antibody (ab67991) at 1/500 dilution

All lanes:

rat brain tissue lysate at 25 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TUFM.

P49411

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUFM also known as elongation factor Tu mitochondrial (EF-Tu) is a protein involved in the protein synthesis process within mitochondria. It has a molecular mass of approximately 50 kDa. TUFM plays an essential role in the translation step of protein synthesis by binding aminoacyl-tRNA and positioning it on the ribosome. Researchers have identified the protein as highly expressed in tissues with high energy demand such as the heart brain and skeletal muscle.

Biological function summary

TUFM assists in mitochondrial protein synthesis which is necessary for the proper function and maintenance of the respiratory chain complexes. It forms part of the mitochondrial ribosome complex operating alongside other elongation factors like EF-G. TUFM’s activity is essential for the production of proteins encoded by mitochondrial DNA which are they key components of oxidative phosphorylation machinery.

Pathways

TUFM integrates into the mitochondrial translation pathway which supports the production of proteins critical for the electron transport chain. This pathway is important for ATP production through oxidative phosphorylation. TUFM interacts with other components such as MRPS18 and MRPL3 to mediate the elongation cycle during mitochondrial protein synthesis. These relationships are essential for coordinating mitochondrial function and bioenergetics.

TUFM mutations or disruptions have connections to mitochondrial disorders such as MELAS syndrome (Mitochondrial Encephalomyopathy Lactic Acidosis and Stroke-like episodes) which affects energy production. The protein's dysfunction can also relate to Parkinson's Disease where impaired mitochondrial activity plays a role. TUFM’s interaction with other mitochondrial proteins like NDUFS1 part of complex I of the electron transport chain underlines its potential impact in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Participates in mitochondrial translation (By similarity). Also plays a role in the regulation of autophagy and innate immunity (PubMed : 22749352, PubMed : 28407488). Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway (PubMed : 28407488). In turn, inhibits RLR-mediated type I interferon while promoting autophagy (PubMed : 22749352).

See full target information TUFM

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:5546 PubMed30944385

2019

GRSF1 is an age-related regulator of senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Jeong Kim,Maria Chun,Junxiang Wan,Changhan Lee,Kelvin Yen,Pinchas Cohen

Experimental gerontology 121:62-70 PubMed30928679

2019

Mitochondrial mRNA translation initiation contributes to oxidative metabolism in the myocardia of aged, obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

David E Lee,Richard A Perry,Jacob L Brown,Megan E Rosa-Caldwell,Lemuel A Brown,Wesley S Haynie,Narasimhan Rajaram,Tyrone A Washington,Nicholas P Greene

Acta physiologica (Oxford, England) 218:167-177 PubMed27061106

2016

Translational machinery of mitochondrial mRNA is promoted by physical activity in Western diet-induced obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

D E Lee,J L Brown,M E Rosa,L A Brown,R A Perry,T A Washington,N P Greene

Immunity 36:933-46 PubMed22749352

2012

The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Lei,Haitao Wen,Yanbao Yu,Debra J Taxman,Lu Zhang,Douglas G Widman,Karen V Swanson,Kwun-Wah Wen,Blossom Damania,Chris B Moore,Patrick M Giguère,David P Siderovski,John Hiscott,Babak Razani,Clay F Semenkovich,Xian Chen,Jenny P-Y Ting

Journal of proteomics 73:1551-61 PubMed20394843

2010

Sub-proteomic fractionation, iTRAQ, and OFFGEL-LC-MS/MS approaches to cardiac proteomics.

Applications

Species

Rat

Chad M Warren,David L Geenen,Donald L Helseth,Hua Xu,R John Solaro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TUFM antibody

Western blot - Anti-TUFM antibody (AB67991)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise