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Rabbit Recombinant Monoclonal TUFM antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854), expandable thumbnail
  • Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Predicted
Predicted
Predicted
Not recommended
Predicted
Rat
Predicted
Predicted
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.

Alternative names

EF-Tu, P43, TUFM

Recommended products

Rabbit Recombinant Monoclonal TUFM antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR12796(B)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249854 is the carrier-free version of Anti-TUFM antibody [EPR12796(B)] ab175199.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TUFM also known as elongation factor Tu mitochondrial (EF-Tu) is a protein involved in the protein synthesis process within mitochondria. It has a molecular mass of approximately 50 kDa. TUFM plays an essential role in the translation step of protein synthesis by binding aminoacyl-tRNA and positioning it on the ribosome. Researchers have identified the protein as highly expressed in tissues with high energy demand such as the heart brain and skeletal muscle.

Biological function summary

TUFM assists in mitochondrial protein synthesis which is necessary for the proper function and maintenance of the respiratory chain complexes. It forms part of the mitochondrial ribosome complex operating alongside other elongation factors like EF-G. TUFM’s activity is essential for the production of proteins encoded by mitochondrial DNA which are they key components of oxidative phosphorylation machinery.

Pathways

TUFM integrates into the mitochondrial translation pathway which supports the production of proteins critical for the electron transport chain. This pathway is important for ATP production through oxidative phosphorylation. TUFM interacts with other components such as MRPS18 and MRPL3 to mediate the elongation cycle during mitochondrial protein synthesis. These relationships are essential for coordinating mitochondrial function and bioenergetics.

Associated diseases and disorders

TUFM mutations or disruptions have connections to mitochondrial disorders such as MELAS syndrome (Mitochondrial Encephalomyopathy Lactic Acidosis and Stroke-like episodes) which affects energy production. The protein's dysfunction can also relate to Parkinson's Disease where impaired mitochondrial activity plays a role. TUFM’s interaction with other mitochondrial proteins like NDUFS1 part of complex I of the electron transport chain underlines its potential impact in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854), expandable thumbnail

    Western blot - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854)

    This data was developed using Anti-TUFM antibody [EPR12796(B)] ab175199, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TUFM antibody [EPR12796(B)] (Anti-TUFM antibody [EPR12796(B)] ab175199) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: A431 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854)

    This data was developed using Anti-TUFM antibody [EPR12796(B)] ab175199, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified Anti-TUFM antibody [EPR12796(B)] ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry (Intracellular) - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854)

    This data was developed using Anti-TUFM antibody [EPR12796(B)] ab175199, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified Anti-TUFM antibody [EPR12796(B)] ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854), expandable thumbnail

    Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854)

    This data was developed using Anti-TUFM antibody [EPR12796(B)] ab175199, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using Anti-TUFM antibody [EPR12796(B)] ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (Anti-TUFM antibody [EPR12796(B)] ab175199)

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (ab249854)

    This data was developed using Anti-TUFM antibody [EPR12796(B)] ab175199, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling TUFM with Anti-TUFM antibody [EPR12796(B)] ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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