Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854)

This data was developed using ab175199, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling TUFM (red) with purified ab175199 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor^®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854)

This data was developed using ab175199, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854)

This data was developed using ab175199, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) cells labelling TUFM with purified ab175199 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

• IP

Supplier Data

Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854)

This data was developed using ab175199, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TUFM antibody [EPR12796(B)] (<a href='/en-us/products/primary-antibodies/tufm-antibody-epr12796b-ab175199'>ab175199</a>)

Predicted band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-TUFM antibody [EPR12796(B)] - BSA and Azide free (AB249854)

This data was developed using ab175199, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TUFM antibody [EPR12796(B)] (<a href='/en-us/products/primary-antibodies/tufm-antibody-epr12796b-ab175199'>ab175199</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 50 kDa

false