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AB317138

Anti-TUFM antibody [HL2117]

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Rabbit Recombinant Monoclonal TUFM antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human TUFM.

View Alternative Names

EF-Tu, P43, TUFM

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [HL2117] (AB317138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [HL2117] (AB317138)

ab317138 detects TUFM protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded mouse heart. TUFM stained by ab317138 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [HL2117] (AB317138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [HL2117] (AB317138)

ab317138 detects TUFM protein at mitochondria by immunohistochemical analysis. Sample : Paraffin-embedded rat kidney. TUFM stained by ab317138 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-TUFM antibody [HL2117] (AB317138)
  • WB

Supplier Data

Western blot - Anti-TUFM antibody [HL2117] (AB317138)

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317138 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-TUFM antibody [HL2117] (ab317138) at 1/5000 dilution

All lanes:

Mouse heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-TUFM antibody [HL2117] (AB317138)
  • WB

Supplier Data

Western blot - Anti-TUFM antibody [HL2117] (AB317138)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317138 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-TUFM antibody [HL2117] (ab317138) at 1/5000 dilution

All lanes:

Rat muscle tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-TUFM antibody [HL2117] (AB317138)
  • WB

Supplier Data

Western blot - Anti-TUFM antibody [HL2117] (AB317138)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317138 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-TUFM antibody [HL2117] (ab317138) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2117

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Zebrafish

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TUFM.

P49411

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Proteins & Peptides

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUFM also known as elongation factor Tu mitochondrial (EF-Tu) is a protein involved in the protein synthesis process within mitochondria. It has a molecular mass of approximately 50 kDa. TUFM plays an essential role in the translation step of protein synthesis by binding aminoacyl-tRNA and positioning it on the ribosome. Researchers have identified the protein as highly expressed in tissues with high energy demand such as the heart brain and skeletal muscle.
Biological function summary

TUFM assists in mitochondrial protein synthesis which is necessary for the proper function and maintenance of the respiratory chain complexes. It forms part of the mitochondrial ribosome complex operating alongside other elongation factors like EF-G. TUFM’s activity is essential for the production of proteins encoded by mitochondrial DNA which are they key components of oxidative phosphorylation machinery.

Pathways

TUFM integrates into the mitochondrial translation pathway which supports the production of proteins critical for the electron transport chain. This pathway is important for ATP production through oxidative phosphorylation. TUFM interacts with other components such as MRPS18 and MRPL3 to mediate the elongation cycle during mitochondrial protein synthesis. These relationships are essential for coordinating mitochondrial function and bioenergetics.

TUFM mutations or disruptions have connections to mitochondrial disorders such as MELAS syndrome (Mitochondrial Encephalomyopathy Lactic Acidosis and Stroke-like episodes) which affects energy production. The protein's dysfunction can also relate to Parkinson's Disease where impaired mitochondrial activity plays a role. TUFM’s interaction with other mitochondrial proteins like NDUFS1 part of complex I of the electron transport chain underlines its potential impact in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Participates in mitochondrial translation (By similarity). Also plays a role in the regulation of autophagy and innate immunity (PubMed : 22749352, PubMed : 28407488). Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway (PubMed : 28407488). In turn, inhibits RLR-mediated type I interferon while promoting autophagy (PubMed : 22749352).
See full target information TUFM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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