AB204234

Anti-Tuftelin 1 antibody

Name: Anti-Tuftelin 1 antibody (ab204234) | Abcam
Brand: Abcam Limited
SKU: AB204234

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(1 Publication)

Rabbit Polyclonal Tuftelin 1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUFT1 aa 100-200.

View Alternative Names

Tuftelin, TUFT1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.

Moderate cytoplasmic positivity in trophoblastic cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-Tuftelin 1 antibody (AB204234)

Immunocytochemical analysis of A431 cells labeling Tuftelin 1 with ab204234 at 4 μg/mL.

Fixation/Permeabilization : PFA/Triton X-100.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.

Moderate cytoplasmic positivity in glandular cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.

Moderate cytoplasmic positivity in squamous epithelial cells.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tuftelin 1 with ab204234 at 1/50 dilution.

Strong cytoplasmic positivity in cells in tubules.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TUFT1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NNX1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuftelin 1 also known as TUFT1 is a protein involved in the mineralization of dental enamel. It holds an approximate molecular mass of 43 kDa and expresses primarily in the ameloblasts the enamel-forming cells of teeth. TUFT1 plays an important mechanical role as a scaffold in the growth and organization of enamel crystals. The protein's expression also appears in other tissues at lower levels though its function outside enamel formation remains less defined.

Biological function summary

Tuftelin 1 contributes to early stages of dental enamel development. It acts as a matrix protein regulating crystal nucleation and growth within the enamel matrix. TUFT1 does not work as an isolated entity but is part of the broader amelogenesis complex interacting with other enamel matrix proteins. It serves a significant biological purpose influencing enamel's hardness and resilience by guiding mineral deposition.

Pathways

TUFT1 interacts closely with enamel-specific pathways and neurodevelopmental processes. In the context of these pathways TUFT1 supports enamel mineralization through interactions with proteins like amelogenin which regulates mineral deposition and enamel maturation. TUFT1 additionally connects with signaling pathways influencing neurodevelopment suggesting a broader range of regulatory activities influencing tissue development.

Tuftelin 1 is implicated in the condition amelogenesis imperfecta a genetic disorder affecting dental enamel development and quality. Mutations in TUFT1 or its associated proteins such as amelogenin lead to enamel defects. Research also suggests links between TUFT1 expression and neurodevelopmental disorders though these associations are still under investigation. Understanding TUFT1's role in these conditions may provide insight into broader implications for developmental biology and genetic pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in the structural organization of the epidermis (PubMed : 36689522). Involved in the mineralization and structural organization of enamel.

See full target information TUFT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 34:e70137 PubMed40619834

2025

New Methodological Approach to Follow the Re-Epithelialisation Phase in the Wound Healing Process on a 3D Full Thickness Skin Model.

Applications

Unspecified application

Species

Unspecified reactive species

Bénédicte Fallou,Mylène Pelleter,Florence Innamorato,Mickaël Roche,Michel Bataillon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tuftelin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunocytochemistry - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuftelin 1 antibody (AB204234)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise