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AB250787

Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free

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Rabbit Recombinant Monoclonal Tuftelin 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Tuftelin, TUFT1

3 Images
Western blot - Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free (AB250787)
  • WB

Supplier Data

Western blot - Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free (AB250787)

This data was developed using ab184949, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tuftelin 1 antibody [EPR13960] (<a href='/en-us/products/primary-antibodies/tuftelin-1-antibody-epr13960-ab184949'>ab184949</a>) at 1/5000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Western blot - Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free (AB250787)
  • WB

Supplier Data

Western blot - Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free (AB250787)

This data was developed using ab184949, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tuftelin 1 antibody [EPR13960] (<a href='/en-us/products/primary-antibodies/tuftelin-1-antibody-epr13960-ab184949'>ab184949</a>) at 1/1000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Western blot - Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free (AB250787)
  • WB

Supplier Data

Western blot - Anti-Tuftelin 1 antibody [EPR13960] - BSA and Azide free (AB250787)

This data was developed using ab184949, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tuftelin 1 antibody [EPR13960] (<a href='/en-us/products/primary-antibodies/tuftelin-1-antibody-epr13960-ab184949'>ab184949</a>) at 1/1000 dilution

All lanes:

293 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa,44 kDa,61 kDa

Observed band size: 11 kDa,65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13960

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250787 is the carrier-free version of ab184949.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuftelin 1 also known as TUFT1 is a protein involved in the mineralization of dental enamel. It holds an approximate molecular mass of 43 kDa and expresses primarily in the ameloblasts the enamel-forming cells of teeth. TUFT1 plays an important mechanical role as a scaffold in the growth and organization of enamel crystals. The protein's expression also appears in other tissues at lower levels though its function outside enamel formation remains less defined.
Biological function summary

Tuftelin 1 contributes to early stages of dental enamel development. It acts as a matrix protein regulating crystal nucleation and growth within the enamel matrix. TUFT1 does not work as an isolated entity but is part of the broader amelogenesis complex interacting with other enamel matrix proteins. It serves a significant biological purpose influencing enamel's hardness and resilience by guiding mineral deposition.

Pathways

TUFT1 interacts closely with enamel-specific pathways and neurodevelopmental processes. In the context of these pathways TUFT1 supports enamel mineralization through interactions with proteins like amelogenin which regulates mineral deposition and enamel maturation. TUFT1 additionally connects with signaling pathways influencing neurodevelopment suggesting a broader range of regulatory activities influencing tissue development.

Tuftelin 1 is implicated in the condition amelogenesis imperfecta a genetic disorder affecting dental enamel development and quality. Mutations in TUFT1 or its associated proteins such as amelogenin lead to enamel defects. Research also suggests links between TUFT1 expression and neurodevelopmental disorders though these associations are still under investigation. Understanding TUFT1's role in these conditions may provide insight into broader implications for developmental biology and genetic pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the structural organization of the epidermis (PubMed : 36689522). Involved in the mineralization and structural organization of enamel.
See full target information TUFT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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