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Rabbit Recombinant Monoclonal TUG antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Tested
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10000 - 1/50000

Notes

-

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT.

Alternative names

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Rabbit Recombinant Monoclonal TUG antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR8615

Purification technique

Affinity purification Protein A

Dissociation constant

2.77 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

TUG regulates glucose homeostasis and energy balance. It forms a complex with GLUT4-containing vesicles controlling their movement to the plasma membrane in response to insulin stimulation. When insulin is present TUG undergoes proteolytic cleavage releasing GLUT4 so that it can facilitate glucose uptake into cells. This regulation of glucose transport is essential in maintaining normal blood glucose levels.

Activity summary

Tether containing a UBX domain for GLUT4 (TUG) also known as ASPSCR1 is a protein that plays a role in cellular trafficking. TUG weighs approximately 67 kDa. It is predominantly expressed in adipose tissue and muscle. TUG acts as an inhibitor that helps regulate the recycling and translocation of the glucose transporter type 4 (GLUT4) by tethering it in the cytoplasm until stimulated by insulin.

Pathways

TUG operates within the insulin signaling pathway and glucose transport pathway. By interacting with GLUT4 TUG controls its translocation important for insulin-mediated glucose uptake. Another significant pathway involving TUG is the trafficking of proteins through the Golgi apparatus where TUG might interact with other UBX domain-containing proteins to manage vesicular traffic.

Associated diseases and disorders

TUG is linked to type 2 diabetes and insulin resistance. Abnormalities in TUG function or expression can disrupt normal GLUT4 trafficking and impair glucose uptake contributing to these metabolic disorders. Furthermore TUG might interact with proteins related to Alzheimer's disease such as those impacting insulin signaling suggesting a potential role in the pathophysiology of neurodegenerative disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-TUG antibody [EPR8615] (ab131237), expandable thumbnail

    Western blot - Anti-TUG antibody [EPR8615] (ab131237)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: TUG knockout HAP1 cell lysate (20 μg)
    Lane 3: NIH/3T3 cell lysate (20 μg)
    Lane 4: HEK293 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-TUG antibody [EPR8615] (AB131237)

    Predicted band size: 60 kDa, 69 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUG antibody [EPR8615] (ab131237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUG antibody [EPR8615] (ab131237)

    ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-TUG antibody [EPR8615] (ab131237), expandable thumbnail

    Western blot - Anti-TUG antibody [EPR8615] (ab131237)

    All lanes: Western blot - Anti-TUG antibody [EPR8615] (AB131237) at 1/10000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: SW480 cell lysate at 10 µg

    Predicted band size: 60 kDa

  • OI-RD Scanning - Anti-TUG antibody [EPR8615] (ab131237), expandable thumbnail

    OI-RD Scanning - Anti-TUG antibody [EPR8615] (ab131237)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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