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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal TUG antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | Flow Cyt | WB | |
---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Tested |
Mouse | Expected | Not recommended | Not recommended | Tested |
Rat | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT.
Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, UBXD9, TUG, UBXN9, RCC17, ASPL, ASPSCR1
Rabbit Recombinant Monoclonal TUG antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9, UBXD9, TUG, UBXN9, RCC17, ASPL, ASPSCR1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
EPR8615
Affinity purification Protein A
2.77 x 10-11 M
Blue Ice
-20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
TUG regulates glucose homeostasis and energy balance. It forms a complex with GLUT4-containing vesicles controlling their movement to the plasma membrane in response to insulin stimulation. When insulin is present TUG undergoes proteolytic cleavage releasing GLUT4 so that it can facilitate glucose uptake into cells. This regulation of glucose transport is essential in maintaining normal blood glucose levels.
Tether containing a UBX domain for GLUT4 (TUG) also known as ASPSCR1 is a protein that plays a role in cellular trafficking. TUG weighs approximately 67 kDa. It is predominantly expressed in adipose tissue and muscle. TUG acts as an inhibitor that helps regulate the recycling and translocation of the glucose transporter type 4 (GLUT4) by tethering it in the cytoplasm until stimulated by insulin.
TUG operates within the insulin signaling pathway and glucose transport pathway. By interacting with GLUT4 TUG controls its translocation important for insulin-mediated glucose uptake. Another significant pathway involving TUG is the trafficking of proteins through the Golgi apparatus where TUG might interact with other UBX domain-containing proteins to manage vesicular traffic.
TUG is linked to type 2 diabetes and insulin resistance. Abnormalities in TUG function or expression can disrupt normal GLUT4 trafficking and impair glucose uptake contributing to these metabolic disorders. Furthermore TUG might interact with proteins related to Alzheimer's disease such as those impacting insulin signaling suggesting a potential role in the pathophysiology of neurodegenerative disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: TUG knockout HAP1 cell lysate (20 μg)
Lane 3: NIH/3T3 cell lysate (20 μg)
Lane 4: HEK293 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TUG antibody [EPR8615] (AB131237)
Predicted band size: 60 kDa, 69 kDa
ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-TUG antibody [EPR8615] (AB131237) at 1/10000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: SW480 cell lysate at 10 µg
Predicted band size: 60 kDa
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com