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AB246970

Anti-TUSC2/FUS1 antibody

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(1 Publication)

Rabbit Polyclonal TUSC2/FUS1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TUSC2 aa 1 to C-terminus.

View Alternative Names

C3orf11, FUS1, LGCC, PDAP2, TUSC2, Tumor suppressor candidate 2, Fusion 1 protein, PDGFA-associated protein 2, Fus-1 protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TUSC2/FUS1 antibody (AB246970)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TUSC2/FUS1 antibody (AB246970)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TUSC2/FUS1 (green) using ab246970 at 4 μg/ml in ICC/IF.

Western blot - Anti-TUSC2/FUS1 antibody (AB246970)
  • WB

Unknown

Western blot - Anti-TUSC2/FUS1 antibody (AB246970)

All lanes:

Western blot - Anti-TUSC2/FUS1 antibody (ab246970) at 0.4 µg/mL

Lane 1:

Vextor only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HEK-293T overexpressing TUSC2/FUS1 with a C-terminal myc-DDK tag, whole cell lysate.

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TUSC2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75896

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TUSC2/FUS1 protein also known by its alternate name TUSC2 plays an essential role in cellular processes. It has a molecular mass of approximately 12 kDa and is found throughout various tissues but is predominantly expressed in the mitochondria. TUSC2 localizes to chromosomal region 3p21.3 which often undergoes deletions in lung cancer. Its expression can be lost or significantly reduced in certain cancers indicating its potential tumor-suppressive role.
Biological function summary

TUSC2 participates in several critical cellular functions and is part of a larger complex with other proteins. It influences cell proliferation apoptosis and signal transduction pathways. TUSC2 functions as a tumor suppressor helping to regulate cell growth and prevent malignant transformation. It acts in apoptosis induction and cell cycle control often by modulating reactive oxygen species (ROS) levels within cells.

Pathways

TUSC2 is instrumental in regulating several cellular pathways. It is a participant in the PI3K/Akt and MAPK pathways which are vital for cell survival growth and metabolism. TUSC2 interacts with proteins like Akt and p53 playing an important role in maintaining cellular homeostasis and preventing aberrant cell division. These interactions help TUSC2 modulate apoptosis and promote cell-cycle arrest under stressful conditions.

TUSC2 has a notable connection to cancer particularly lung cancer and mesothelioma. In these diseases TUSC2 expression often decreases due to chromosomal deletions. The protein's interaction with other tumor suppressors such as p53 and PTEN influences tumorigenesis by disrupting normal signaling pathways leading to uncontrolled cell proliferation. Restoring TUSC2 function or expression could open avenues for therapeutic interventions in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a tumor suppressor, inhibiting colony formation, causing G1 arrest and ultimately inducing apoptosis in homozygous 3p21.3 120-kb region-deficient cells.
See full target information TUSC2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed31973107

2020

The TUSC2 Tumour Suppressor Inhibits the Malignant Phenotype of Human Thyroid Cancer Cells via SMAC/DIABLO Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Raffaela Mariarosaria Mariniello,Francesca Maria Orlandella,Anna Elisa De Stefano,Paola Lucia Chiara Iervolino,Giovanni Smaldone,Neila Luciano,Nara Cervone,Francesco Munciguerra,Silvia Esposito,Peppino Mirabelli,Giuliana Salvatore
View all publications
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