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Rabbit Polyclonal TUSC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TUSC3 conjugated to Keyhole Limpet Haemocyanin.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human TUSC3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13454
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Cow, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

-

Predicted
Predicted

Species

Cow, Monkey

Dilution info

-

Notes

-

Target data

Function

Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues; in this function seems to act redundantly with MAGT1. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.Magnesium transporter.

Alternative names

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Rabbit Polyclonal TUSC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TUSC3 conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human TUSC3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13454
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

The protein involves itself in an important role in the cellular mechanism of N-glycosylation. TUSC3 associates with the oligosaccharyltransferase complex facilitating the transfer of oligosaccharides to nascent proteins. This process enhances the stability and function of many proteins that require glycosylation. TUSC3's proper activity ensures the correct folding and functionality of these glycoproteins impacting cell homeostasis and viability.

Activity summary

TUSC3 also known as Tumor Suppressor Candidate 3 is a protein weighing approximately 42 kDa. It plays a role in N-glycosylation an important post-translational modification mechanism. TUSC3 is mainly expressed in the human endoplasmic reticulum and is involved in protein folding processes. By aiding in this process TUSC3 supports the correct functioning of proteins throughout the cell. Within the endoplasmic reticulum it serves as part of a complex that ensures proper protein maturation.

Pathways

TUSC3 functions within both the N-glycosylation pathway and the unfolded protein response (UPR). Its activity in N-glycosylation ensures proteins are correctly folded and functional while its role in the UPR helps manage cellular stress by promoting the proper folding of proteins under stress conditions. TUSC3 works closely with proteins like calnexin in these pathways collaborating to maintain protein quality control and reduce potential cellular dysfunction.

Associated diseases and disorders

TUSC3 links to certain cancers and neurological conditions. Its role as a tumor suppressor implicates it in various cancers where reduced expression can lead to tumor progression. Additionally mutations or dysfunctions in TUSC3 have been associated with intellectual disabilities where glycosylation deficiencies contribute to disease pathology. TUSC3 interacts with proteins such as alpha-dystroglycan in these contexts where alterations can disrupt normal cellular functions and lead to disease development or progression.

Product promise

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Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-TUSC3 antibody (ab230520), expandable thumbnail

    Western blot - Anti-TUSC3 antibody (ab230520)

    All lanes: Western blot - Anti-TUSC3 antibody (AB230520) at 1/500 dilution

    Lane 1: SK-BR-3 (Human mammary gland adenocarcinoma cell line)

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma)

    Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line)

    Lane 4: MDA-MB-231 (Human breast adenocarcinoma cell line)

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUSC3 antibody (ab230520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUSC3 antibody (ab230520)

    Formalin-fixed, paraffin-embedded human colon cancer tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ab230520 at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry/ Immunofluorescence - Anti-TUSC3 antibody (ab230520), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TUSC3 antibody (ab230520)

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for TUSC3 using ab230520 at a dilution of 1/100 in ICC/IF.

    Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUSC3 antibody (ab230520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUSC3 antibody (ab230520)

    Formalin-fixed, paraffin-embedded human testis tissue stained for TUSC3 with ab230520 at a 1/100 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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