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Rat Recombinant Monoclonal ENV antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Transfected cell line - Bird samples.

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Images

Western blot - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Rat
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow Cyt (Intra)
Bird
Predicted
Predicted
Predicted
Predicted
Transfected cell line - Bird
Tested
Tested
Tested
Tested

Tested
Tested

Species
Transfected cell line - Bird
Dilution info
-
Notes

-

Predicted
Predicted

Species
Bird
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Bird
Dilution info
-
Notes

-

Predicted
Predicted

Species
Bird
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Bird
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Bird
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Bird
Dilution info
-
Notes

-

Predicted
Predicted

Species
Bird
Dilution info
-
Notes

-

Target data

Function

Responsible for susceptibility to the retrovirus subgroup A Rous sarcoma virus.

Alternative names

Subgroup A Rous sarcoma virus receptor pg950, Low density lipoprotein receptor-related protein, Tva

Recommended products

Rat Recombinant Monoclonal ENV antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Transfected cell line - Bird samples.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
PICA187E
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab271302 is the carrier-free version of Anti-TVA antibody [PICA187E] ab271292.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Tumor Virus A Receptor (TVA) often referred to as Tva or LDLR family member 1 (LDLR1) is a protein that acts as a receptor for specific avian sarcoma and leukosis viruses (ASLV) subgroup A. It has a molecular mass of approximately 47 kDa. TVA is expressed on the surface of cells in various tissues playing a role in viral entry. It belongs to the low-density lipoprotein receptor (LDLR) family which is involved in endocytosis and ligand binding processes.

Biological function summary

TVA acts as part of the viral entry complex facilitating the binding and internalization of certain retroviruses. Through its function as a receptor TVA allows the virus to attach to and penetrate host cells initiating infection. The protein’s role in viral entry makes it important for viral lifecycle progression. TVA does not function in isolation but often works in tandem with other receptors to mediate efficient viral uptake into the host cell.

Pathways

TVA participates in the endocytosis pathway which is integral for the uptake of viruses into cells. This pathway includes other proteins such as clathrin and dynamin which play roles in vesicle formation and membrane scission. TVA's interaction with these components facilitates the internalization and trafficking of viral particles within the host cell aiding in the infection process. The protein is also related to signal transduction pathways that might be hijacked by viruses to sustain their replication inside host cells.

Associated diseases and disorders

The role of TVA in viral infections positions it as a critical factor in understanding certain leukemias and sarcomas caused by ASLV. The infection process mediated by TVA can lead to cellular transformation and tumorigenesis particularly in avian species. While direct associations with human diseases have not been extensively documented studying TVA's interaction with viral glycoproteins provides insight into similar mechanisms in other retroviral infections including those involving related receptors like LDLR.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Western blot - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TVA antibody [PICA187E] (Anti-TVA antibody [PICA187E] ab271292) at 1/5000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/50000 dilution

    Observed band size: 25-37 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T transfected with a TVA construct and (Panel B) HEK-293T transfected with empty plasmid, labeling TVA with Anti-TVA antibody [PICA187E] ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on (Panel A) HEK-293T transfected with a TVA construct, no staining on (Panel B) HEK-293T transfected with empty plasmid. The section was incubated with Anti-TVA antibody [PICA187E] ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells transfected with myc-tagged TVA expression vector labelling TVA with Anti-TVA antibody [PICA187E] ab271292 at 1/50 (21.96 μg/ml) dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged TVA expression vector. Myc-Tag mouse mAb (Alexa Fluor® 647) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Used PBS instead of Anti-TVA antibody [PICA187E] ab271292, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEH-293T (human embryonic kidney epithelial cell) (transfected with myc tagged TVA construct) cells labelling TVA with Anti-TVA antibody [PICA187E] ab271292 at 1/1000 dilution (0.1μg) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F (ab)2 Anti-Rat IgG Fc (Alexa Fluor®488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161)was used as the secondary antibody at a 1/2000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TVA with Anti-TVA antibody [PICA187E] ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Negative control: No staining on human cerebrum. The section was incubated with Anti-TVA antibody [PICA187E] ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TVA with Anti-TVA antibody [PICA187E] ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Negative control: No staining on mouse cerebrum. The section was incubated with Anti-TVA antibody [PICA187E] ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using Anti-TVA antibody [PICA187E] ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TVA with abab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Negative control: No staining on rat cerebrum. The section was incubated with Anti-TVA antibody [PICA187E] ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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