Anti-TVA antibody [PICA187E] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells transfected with myc-tagged TVA expression vector labelling TVA with ab271292 at 1/50 (21.96 μg/ml) dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged TVA expression vector. Myc-Tag mouse mAb (Alexa Fluor^® 647) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Used PBS instead of ab271292, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control : No staining on human cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEH-293T (human embryonic kidney epithelial cell) (transfected with myc tagged TVA construct) cells labelling TVA with ab271292 at 1/1000 dilution (0.1μg) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F (ab)2 Anti-Rat IgG Fc (Alexa Fluor^®488, ab150161)was used as the secondary antibody at a 1/2000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T transfected with a TVA construct and (Panel B) HEK-293T transfected with empty plasmid, labeling TVA with ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on (Panel A) HEK-293T transfected with a TVA construct, no staining on (Panel B) HEK-293T transfected with empty plasmid. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control : No staining on mouse cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 μg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control : No staining on rat cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-TVA antibody [PICA187E] - BSA and Azide free (AB271302)

This data was developed using ab271292 the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TVA antibody [PICA187E] (<a href='/en-us/products/primary-antibodies/tva-antibody-pica187e-ab271292'>ab271292</a>) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/50000 dilution

Observed band size: 25-37 kDa

false

Exposure time: 10s